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Merck

A1602

Sigma-Aldrich

Aminoglucosidasa from Aspergillus niger

ammonium sulfate suspension, ≥40 units/mg protein

Sinónimos:

1,4-α-D-Glucano glucohidrolasa, Exo-1,4-α-glucosidasa, Glucoamilasa

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About This Item

Número de CAS:
Comisión internacional de enzimas:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

ammonium sulfate suspension

specific activity

≥40 units/mg protein

shipped in

wet ice

storage temp.

2-8°C

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General description

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Unit Definition

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae
Teets NM, et al.
The Journal of Experimental Biology, 214(5), 806-814 (2011)
Christina Pedersen et al.
PloS one, 13(3), e0194468-e0194468 (2018-03-21)
Insulin is a vital part of diabetes treatment, whereas glucagon is primarily used to treat insulin-induced hypoglycemia. However, glucagon is suggested to have a central role in the regulation of body weight, which would be beneficial for diabetic patients. Since
A method for routine measurements of total sugar and starch content in woody plant tissues
Chow PS and Landhausser SM
Tree physiology, 24(10), 1129-1136 (2004)
Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries, 183-183 (2000)
Hugh S Winwood-Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)
Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores

Artículos

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolos

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

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