62314
Lipase Substrate
for the titrimetric determination of enzyme activity
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About This Item
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grade
for the titrimetric determination of enzyme activity
form
liquid
technique(s)
titration: suitable
refractive index
n20/D 1.36
density
1.05 g/mL at 20 °C
storage temp.
2-8°C
Physical form
aqueous solution with 4.5 mM triolein; 1 M NaCl; 13% (w/v) Triton™ X-100
Other Notes
Assay of microbial lipases with emulsified trioleoyl glycerol; the fatty acids released are titrated with a pH stat
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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A new assay of microbial lipases with emulsified trioleoyl glycerol.
Analytical biochemistry, 112(2), 219-222 (1981-04-01)
Chembiochem : a European journal of chemical biology, 6(10), 1776-1781 (2005-08-12)
Lipases and esterases are responsible for carboxylester hydrolysis inside and outside cells and are useful biocatalysts for (stereo)selective modification of synthetic substrates. Here we describe novel fluorescent suicide inhibitors that differ in structure and polarity for screening and discrimination of
Free radical research, 23(4), 317-327 (1995-10-01)
We report on a new method for the determination of lipid oxidation in lipoproteins and plasma. The biological lipid system is preloaded with a fluorescent analog of phosphatidylcholine containing diphenylhexatriene (DPH) propionic acid covalently linked to the sn-2 position. When
Analytical biochemistry, 276(1), 72-80 (1999-12-10)
We report on the determination of active enzyme components in pure and crude lipases, using fluorescent inhibitors for covalent modification and visualization of the enzymatically active proteins. Lipase-specific compounds are triacylglycerol analogs, namely 1,2(2, 3)-di-O-alkylglyceroalkylphosphonic acid-p-nitrophenyl esters, containing a fluorescent
Chembiochem : a European journal of chemical biology, 7(3), 527-534 (2006-02-14)
Protein and small-molecule microarrays are useful tools for high-throughput analysis of DNA-protein, protein-protein, and protein-small molecule interactions. Here we report on novel microarrays for activity screening of lipases and esterases based on phosphonic acid ester inhibitors. These compounds are activity
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