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04511

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Live/Dead Cell Double Staining Kit

suitable for fluorescence

Sinónimos:

Staining kit for live/dead cells

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.32

idoneidad

suitable for fluorescence

Nivel de calidad

100

temp. de almacenamiento

−20°C

Categorías relacionadas

Aplicación

The Live/Dead Cell Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains calcein-AM and propidium iodide (PI) solutions, which stain viable and dead cells, respectively. Calcein-AM, acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Though calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein-AM by esterase in a viable cell emits a strong green fluorescence (λex 490 nm, λem 515 nm). Therefore, calcein-AM only stains viable cells. Alternatively, the nuclei staining dye PI cannot pass through a viable cell membrane. It reaches the nucleus by passing through disordered areas of dead cell membrane, and intercalates with the DNA double helix of the cell to emit red fluorescence (λex 535 nm, λem 617 nm). Since both calcein and PI-DNA can be excited with 490 nm light, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. Using λex 545 nm, only dead cells can be observed.

Solo componentes del kit

Referencia del producto
Descripción
  • Solution A (Calcein AM solution) 4 × 50
  • Solution B (propidium iodide solution) 300 μL

Producto relacionado

Referencia del producto
Descripción
Precios

92210

Timestrip Plus™ 0 °C

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

185.0 °F - closed cup

Punto de inflamabilidad (°C)

85 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
BCCM1249
BCCK6792
BCCJ1064
BCCH3243
BCCG3361

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