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21888

Sigma-Aldrich

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sinónimos:

CFSE

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About This Item

Fórmula empírica (notación de Hill):
C29H19NO11
Número de CAS:
150347-59-4
Peso molecular:
557.46
Número MDL:
MFCD00037466
Código UNSPSC:
12352108
ID de la sustancia en PubChem:
57647951
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Línea del producto

BioReagent

Nivel de calidad

200

Ensayo

≥90% (HPLC)

Formulario

solid

mp

152-154 °C (lit.)

solubilidad

DMF: soluble
DMSO: soluble

fluorescencia

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

idoneidad

suitable for fluorescence

temp. de almacenamiento

−20°C

cadena SMILES

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

Clave InChI

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Categorías relacionadas

Descripción general

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.[1]

Aplicación

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry.[2][3] Forms green fluorescent conjugates on deacetylation.
5[4]-Carboxyfluorescein diacetate N-succinimidyl ester has been used:
  • to label cells for flow cytometry[5][6]
  • in lymphocyte proliferation assay[7][8]
  • to determine the intracellular or intra-organellar pH in chloroplasts[4]
  • to label Leishmania amazonensis and Leishmania major[9]

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

Acciones bioquímicas o fisiológicas

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.[10]

Envase

Bottomless glass bottle. Contents are inside inserted fused cone.

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Referencia del producto
Descripción
Precios

06693

Timestrip Plus™ -20 °C

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
BCCF7480
BCCK2345
BCCC4532
BCBW9190
BCBT5235

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

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M Bronner-Fraser
The Journal of cell biology, 101(2), 610-617 (1985-08-01)
The possible role of a 140-kD cell surface complex in neural crest adhesion and migration was examined using a monoclonal antibody JG22, first described by Greve and Gottlieb (1982, J. Cell. Biochem. 18:221-229). The addition of JG22 to neural crest
Autophagic induction greatly enhances Leishmania major intracellular survival compared to Leishmania amazonensis in CBA/j-infected macrophages
Dias BRS, et al.
Frontiers in Microbiology, 9 (2018)
Lymphoproliferative responses to dendritic cell presentation of sensitizing allergens in atopic children with multiple allergies
Scott-Taylor TH, et al.
Annals of Allergy, Asthma & Immunology, 119(3), 274-283 (2017)
WNT5A transforms intestinal CD8αα+ IELs into an unconventional phenotype with pro-inflammatory features
Zhao D, et al.
BMC Gastroenterology, 15(1), 173-173 (2015)
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe
Su PH and Lai YH
Frontiers in Plant Science, 8, 2079-2079 (2017)
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Artículos

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Questions

1–6 of 6 Questions  

  1. What is the in vivo (mouse) half-life of CFDA-SE? What is the route of clearance (kidney, liver?)

    1 answer

    1. The half-life is not determined for this product.
      Please see the link below to a publication that may be helpful:
      https://www.semanticscholar.org/paper/The-Use-of-Carboxyfluorescein-Diacetate-Ester-to-Quah-Parish/cae3435e063cb9f46cea4ddfc7c560f5821e19b3

      Please note that, unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

      Helpful?

  2. Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, light sensitive?

    1 answer

    1. Yes. The powder and solutions (especially solutions) should be kept protected from ambient light whenever possible.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, fluorescent? Are conjugates of this compound fluorescent?

    1 answer

    1. No. This compound and conjugates of this compound become fluorescent only when acted upon by an enzyme such as esterase. The enzyme removes the acetate groups.

      Helpful?

  5. At what concentration is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, soluble in DMSO?

    1 answer

    1. We have found 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester to be soluble in DMSO at a concentration of 1 mg/mL.

      Helpful?

  6. What is the ratio between the (5) and (6) isomer of Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?

    1 answer

    1. Our Quality Control group does not routinely report the ratio; however, it is very likely that both isomers are present.

      Helpful?

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