MABE1798
Anti-ATRX Antibody
mouse monoclonal, 39F
Sinónimos:
ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein, XNP, Znf-HX, EC:3.6.4.12
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Nombre del producto
Anti-ATRX Antibody, clone 39f, clone 39F, from mouse
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
39F, monoclonal
reactividad de especies
human, mouse
técnicas
immunocytochemistry: suitable
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ATRX(546)
Descripción general
Transcriptional regulator ATRX (UniProt: P46100; also known as EC:3.6.4.12, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein, XNP, Znf-HX, ATRX) is encoded by the ATRX (also known as RAD54L, XH2) gene (Gene ID: 546) in human. ATRX is ubiquitously distributed and is involved in transcriptional regulation and chromatin remodeling. ATRX has a PHD zinc finger motif and an ATPase/helicase domain of the SWI/SNF type. It facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. ATRX binds to DNA tandem repeat sequences in both telomeres and euchromatin and is shown to bind quadruplex structures in vitro. ATRX associates with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with CBX5/HP1 alpha. It interacts with DAXX to form the chromatin remodeling complex ATRX:DAXX, which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres. ATRX is believed to be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. ATRX is phosphorylated at serine residues during mitosis and phosphorylation is most evident at pro-metaphase stage. Phosphorylation of ATRX is necessary for release of the protein from the nuclear matrix and that it may facilitate progression to mitosis. Dephosphorylation of ATRX seems to coincide with exit of the cells from M phase. Mutations in ATRX gene are known to cause severe psychomotor and mental retardation, facial dysmorphism, and urogenital abnormalities. (Ref.: Berube, NG et al. (2000). Hum. Mol. Gen. 9(4):539-547).
Especificidad
Clone 39f specifically recognizes human ARTX. This clone binds to an epitope beetween amino acid 85-319 in the N-terminal half of human ATRX.
Inmunógeno
Recombinant fragment corresponding to 235 amino acids from the N-terminal half of human ARTX.
Aplicación
Anti-ATRX Antibody, clone 39f, Cat. No. MABE1798, is a highly specific mouse monoclonal antibody that targets ATRX and has been tested in Immunocytochemistry and Western Blotting .
Immunocytochemistry Analysis: A representative lot detected ATRX in HeLa cells (McDowell, T.L., et. al. (1999). Proc Natl Acad Sci USA. 96(24):13983-8).
Western Blotting Analysis: A representative lot detected ATRX in EBV-transformed lymphocytes (McDowell, T.L., et. al. (1999). Proc Natl Acad Sci USA. 96(24):13983-8).
Western Blotting Analysis: A representative lot detected ATRX in depletion in fibroblasts (Lukashchuk, V., et. al. (2008). J Virol. 82(24):12543-54).
Western Blotting Analysis: A representative lot detected ATRX in EBV-transformed lymphocytes (McDowell, T.L., et. al. (1999). Proc Natl Acad Sci USA. 96(24):13983-8).
Western Blotting Analysis: A representative lot detected ATRX in depletion in fibroblasts (Lukashchuk, V., et. al. (2008). J Virol. 82(24):12543-54).
Calidad
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected ATRX in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected ATRX in 10 µg of HEK293 cell lysate.
Descripción de destino
~282 kDa observed; 282.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forma física
Format: Purified
Otras notas
Concentration: Please refer to lot specific datasheet.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
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Jennifer Garbarino et al.
Translational oncology, 14(9), 101147-101147 (2021-06-13)
Alpha Thalassemia/Mental Retardation Syndrome X-Linked (ATRX) is mutated frequently in gliomas and represents a potential target for cancer therapies. ATRX is known to function as a histone chaperone that helps incorporate histone variant, H3.3, into the genome. Studies have implicated
Anne-Charlotte Stilp et al.
PLoS pathogens, 18(8), e1010748-e1010748 (2022-08-09)
The chromatin remodeling protein alpha thalassemia/mental retardation syndrome X-linked (ATRX) is a component of promyelocytic leukemia nuclear bodies (PML-NBs) and thereby mediates intrinsic immunity against several viruses including human cytomegalovirus (HCMV). As a consequence, viruses have evolved different mechanisms to
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