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Key Documents

MAB1987Z

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone P4C10, azide free

clone P4C10, Chemicon®, from mouse

Sinónimos:

CD29, MAB1987

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

P4C10, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGB1(3688)

General description

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Specificity

Reacts with the beta-1 subunit of the integrin protein family. Appears to be human specific.Unlike many beta-1 monoclonals 4C10′s epitope is not trypsin sensitive.

Application

Anti-Integrin β1 Antibody, clone P4C10, azide free detects level of Integrin β1 & has been published & validated for use in ELISA, IC, IP, FUNC & WB.
Inhibits attachment of UCLA-P3 cells to collagen type I (Wayner, 1991). Inhibits endodermal and keratinocytes to collagen, fibronectin and laminin. It also inhibits cell to cell adhesion. Typical dilution ranges are 1:50-1:500.

Immunoflourescence

Immunoblotting

Immunoprecipitation

ELISA

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β1 in HeLa cells.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Target description

88 KDa

Linkage

Replaces: 04-1109

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Human Tonsil, human skin, human lung, liver, and skeletal muscle tissues

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Optional

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Steven Mortillo et al.
The Journal of comparative neurology, 520(9), 2041-2052 (2012-04-11)
β1-containing integrins are required for persistent synaptic potentiation in hippocampus and regulate hippocampal-dependent learning. Based largely on indirect evidence, there is a prevailing assumption that β1-integrins are localized at synapses, where they contribute to synapse adhesion and signaling, but this
Geneviève Dufour et al.
The Journal of biological chemistry, 279(42), 44113-44122 (2004-08-10)
We have shown previously that human intestinal epithelial cell survival and anoikis are distinctively regulated according to the state of differentiation. Here we analyzed the roles of protein kinase B/Akt isoforms in such differentiation state distinctions. Anoikis was induced in
Thomas Kaido et al.
Diabetes, 55(10), 2723-2729 (2006-09-28)
The impact of extracellular matrix on insulin production needs to be understood both to optimize the derivation of functional beta-cells for transplantation and to understand mechanisms controlling islet neogenesis and glucose homeostasis. In this study, we present evidence that adhesion
Weisheng Wang et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 41(11), 2723-2732 (2016-06-09)
Estradiol (E2) perfusion rapidly increases the strength of fast excitatory transmission and facilitates long-term potentiation in the hippocampus, two effects likely related to its memory-enhancing properties. Past studies showed that E2's facilitation of transmission involves activation of RhoA signaling leading
Integrin-driven actin polymerization consolidates long-term potentiation.
Kramar, EA; Lin, B; Rex, CS; Gall, CM; Lynch, G
Proceedings of the National Academy of Sciences of the USA null

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