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IPVH85R

Millipore

Immobilon®-P Membrane

1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Sinónimos:

Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031613
NACRES:
NB.23

product name

Membrana Immobilon®-P, PVDF, 0,45 m, rollo de 8,5 cm x 10 m., 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

8.5 cm × 10 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
 215 μg/cm2 adsorption capacity (BSA)
 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

Categorías relacionadas

General description

La membrana de transferencia Immobilon®-P es una membrana microporosa hidrófoba de fluoruro de polivinilideno (PVDF) con un tamaño nominal de poro de 0,45 micras (μm). Se utiliza para la transferencia de proteínas desde una variedad de matrices de gel. La membrana de transferencia Immobilon®-P se recomienda para la mayoría de las inmunoelectrotransferencias, en especial para proteínas superiores a 20 kDa. Esta membrana exhibe características de manejo y capacidades de tinción mejoradas, una relación señal-ruido más elevada para mayor sensibilidad y una mayor resistencia a disolventes, en comparación con la membrana de nitrocelulosa.
Principales mecanismos de adsorción: Electrostático, hidrófobo
Código del filtro: IPVH

Application

La membrana Immobilon®-P, de PVDF, 0,45 μm, rollo de 8,5 cm x 10 m se ha utilizado en análisis de transferencia Western y coinmunoprecipitación.

Linkage

Sustituye a: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Wei Cai et al.
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Neuropathic pain, caused by damage to the nerve system, is one of the most challenging diseases in the world. Moreover, the etiology remains unclear. Circular RNAs (circRNAs) have been revealed to participate in various biological progress, including neuropathic pain. However
Chunzhi Li et al.
Investigative ophthalmology & visual science, 61(10), 9-9 (2020-08-07)
Retinoblastoma (RB) is the most common primary intraocular tumor in children. Chemoresistance is the major obstacle for treatment of these tumors. This study aims to determine whether or not downregulating microRNA-222 (miR-222) could serve as a potential therapeutic target for
microRNA-222-Mediated VHL Downregulation Facilitates Retinoblastoma Chemoresistance by Increasing HIF1? Expression.
Chunzhi Li, et al.
Investigative Ophthalmology & Visual Science (2020)
Jeremiah Tsyporin et al.
Cell reports, 35(12), 109269-109269 (2021-06-24)
Projection neuron subtype identities in the cerebral cortex are established by expressing pan-cortical and subtype-specific effector genes that execute terminal differentiation programs bestowing neurons with a glutamatergic neuron phenotype and subtype-specific morphology, physiology, and axonal projections. Whether pan-cortical glutamatergic and

Artículos

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocolos

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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