I2393

Interleukin-1β from rat

IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : IL-1β, Interleukin-1β from rat, Lymphocyte activating factor
• Numéro MDL: MFCD01094098
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (SDS-PAGE and HPLC)
• Forme: lyophilized powder
• Puissance: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• Poids mol.: 17.3 kDa
• Conditionnement: pkg of 10 and 50 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg
• Numéro d'accès UniProt: Q63264
• Température de stockage: −20°C
• Informations sur le gène: rat ... Il1b(24494)

Description

Description générale

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin-2.¹ IL-1 is primarily released from stimulated macrophages and monocytes, but is also released from several other cell types,² and is thought to play a key role in inflammatory and immune responses.³ Other synonyms for IL-1 include: Endogenous Pyrogen (EP), Mitogenic Protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRF_H), B cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF).⁴ The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence. Recombinant rat IL-1β is a 17.3 kDa protein containing 153 amino acid residues.

Forme physique

Lyophilized from a 0.2 μm filtered buffered solution.

Remarque sur l'analyse

The biological activity is measured in a cell proliferation assay using murine D10S cells.

Formule chimique

1. Gery, I., et al., J. Exp. Med., 136, 128 (1972).
2. Oppenheim, J. J., et al., Immunol. Today, 7, 45 (1986).
3. Durum, S. K., et al., Annu. Rev. Immunol., 3, 263 (1985).
4. Aarden, L., et al., J. Immunol., 123, 2928 (1979).
5. Symons, J., et al., Lymphokines and Interferons, A Practical Approach, Clemens, M., et al., (eds.), IRL Press, Oxford (1987).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)