T5944

Tumor Necrosis Factor-α from rat

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonyme(s) : Cachectin, TNF-α
• Numéro MDL: MFCD00282383
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: rat
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (SDS-PAGE and HPLC)
• Forme: powder
• Qualité: endotoxin tested
• Poids mol.: predicted mol wt ~17 kDa
• Conditionnement: pkg of 10 μg; pkg of 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <1 EU/μgtested (LAL test)
• Couleur: white
• Solubilité: water: soluble
• Numéro d'accès UniProt: P16599
• Température de stockage: −20°C
• Informations sur le gène: rat ... Tnf(24835)

Description

Description générale

The Tnf (tumor necrosis factor) gene is mapped to rat chromosome 20p12.

Application

TNF-α influences the growth and function of both normal and neoplastic cells. TNF-α causes cytolysis or cytostasis of certain transformed cells being synergistic with γ-interferon in its cytotoxicity.

Tumor Necrosis Factor-α from rat has been used:

• To stimulate inducible nitric oxide synthase (iNOS) expression in the macrophages as an indication of M1 macrophage activation
• To study its effect on pancreatic β cell apoptosis
• To evaluate the effect of progesterone on the expression of tumor necrosis factor (TNF)-α in synovial membrane
• As a blocking antigen in the control, for immunohistochemical analysis

Actions biochimiques/physiologiques

Tumor necrosis factor-α (TNF-α), also known as cachectin, plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication, and hematopoiesis. TNF-α is expressed by a variety of cells, with numerous inductive and suppressive agents. It is primarily produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens, and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2.

Forme physique

Lyophilized from an 0.2 μm-filtered buffered solution with bovine serum albumin as a carrier.

Remarque sur l'analyse

The biological activity is measured in a cytotoxic assay using a TNF-susceptible mouse L929 cell line in the presence of actinomycin D.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable