HPLC Analysis of Monoclonal Antibodies (mAb) with Charge Variance on Antibodix^® WCX-NP10

column

Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)

mobile phase

(A) 10 mM sodium phosphate buffer, pH 7.5; (B) A + 100 mM NaCl

gradient

15 to 55% B in 30 min

flow rate

0.8 mL/min

pressure

840 psi (58 bar)

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

monoclonal antibody