HPLC Analysis of mAb-X22 on Antibodix^® WCX-NP10, Impact of Mobile Phase pH

column

Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)

mobile phase

(A) 10 mM sodium phosphate buffer at 7.5 pH; (B), A + 100 mM NaCl at 7.0 pH

gradient

15 to 65% B in 60 min

flow rate

0.8 mL/min

column temp.

25 °C

detector

UV, 214 nm

injection

10 μL

sample

MAb-X22, 1.5 mg/mL