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Documentos clave

SRP6053

Sigma-Aldrich

ProBDNF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Sinónimos:

Abrineurin, BDNF, Brain-derived neurotrophic factor

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

human

recombinante

expressed in E. coli

Ensayo

≥95% (HPLC)
≥95% (SDS-PAGE)

Formulario

lyophilized

mol peso

~52 kDa

envase

pkg of 10 μg

Nº de acceso NCBI

P23560

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... BDNF(627)

Categorías relacionadas

Descripción general

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors.[1][2] The precursor form of brain-derived neurotrophic factor (pro-BDNF) undergoes post-translational cleavage to form mature BDNF (mBDNF). The gene encoding this protein is localized on human chromosome 11p14.1.[3][4]

Acciones bioquímicas o fisiológicas

Precursor form of brain-derived neurotrophic factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) (2) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the tropomyosin-related kinase B (TrkB) kinase receptor and promotes the survival, growth and differentiation of neurons.[1] As proneurotrophins and mature neurotrophins elicit opposite biological effects, pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

Forma física

Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.

Nota de preparación

Centrifuge the vial prior to opening.

Reconstitución

Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.

Otras notas

Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
7K014273
7K194273

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Function and evolution in the NGF family and its receptors.
Ebendal T
Journal of Neuroscience Research, 32(4), 461-470 (1992)
11p14.1 microdeletions associated with ADHD, autism, developmental delay, and obesity.
Shinawi M
American Journal of Medical Genetics. Part A, 155A(6), 1272-1280 (2011)
The presence of a single-nucleotide polymorphism in the BDNF gene affects the rate of locomotor adaptation after stroke.
Helm EE
Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 234(2), 341-351 (2016)
Hao-Yun Zhang et al.
Biochimica et biophysica acta, 1843(12), 2967-2975 (2014-09-23)
Iron accumulation is observed in the substantia nigra of patients with Parkinson's disease. However, it is unknown whether neurotrophic factors, brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) participate in the modulation of neuronal iron metabolism. Here
Guoqi Zhu et al.
British journal of pharmacology, 172(9), 2354-2368 (2015-01-07)
Mild cognitive deficit in early Parkinson's disease (PD) has been widely studied. Here we have examined the effects of memantine in preventing memory deficit in experimental PD models and elucidated some of the underlying mechanisms. I.p. injection of 1-methyl-4- phenyl-1,2,3,6-tetrahydro
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Questions

  1. Hi, Is this recombinant protein functional?

    1 answer

    1. This product is a growth factor analyzed for Appearance, Purity, and Endotoxin level. Kindly review the following citation where this material was used in Primary rat VM neuron culture.

      Zhang HY, Song N, Jiang H, Bi MX, Xie JX. Brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor inhibit ferrous iron influx via divalent metal transporter 1 and iron regulatory protein 1 regulation in ventral mesencephalic neurons. Biochim Biophys Acta. 2014 Dec;1843(12):2967-75. doi: 10.1016/j.bbamcr.2014.09.010. Epub 2014 Sep 16. PMID: 25239763.

      Helpful?

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