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S6322

Sigma-Aldrich

Sigma Adjuvant System®

oil

Sinónimos:

Adjuvant System, Sigma Adjuvant

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1 VIAL
$250.000

$250.000

Fecha estimada de envío08 de mayo de 2025

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1 VIAL
$250.000

About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

$250.000

Fecha estimada de envío08 de mayo de 2025

Solicitar un pedido a granel

Formulario

oil

Nivel de calidad

300

temp. de almacenamiento

2-8°C

Aplicación

Adjuvanticity Assay: EIA titer for test mice is at least 2X greater that that of control mice.

Acciones bioquímicas o fisiológicas

Sigma Adjuvant System is a stable oil-in-water emulsion that may be used as an alternative to the classical Freund′s water-in-oil emulsions. It is derived from bacterial and mycobacterial cell wall components that provide potent stimulus to the immune system.

Forma física

Each vial contains 0.5 mg Monophosphoryl Lipid A (detoxified endotoxin) from Salmonella minnesota and 0.5 mg synthetic Trehalose Dicorynomycolate in 2% oil (squalene)-Tween® 80-water.

Información legal

Sigma Adjuvant System is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H304

Clasificaciones de peligro

Asp. Tox. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000329924
0000235360
0000235360
0000329924
0000245077

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Déirdre B Ní Eidhin et al.
FEMS immunology and medical microbiology, 52(2), 207-218 (2007-12-21)
Clostridium difficile is the leading cause of infectious antibiotic-associated diarrhoea, particularly among the elderly. Its surface-layer protein (SLP) was tested as a vaccine component in a series of immunization and challenge experiments with Golden Syrian hamsters, combined with different systemic
Nadja Thönes et al.
Journal of virology, 82(11), 5472-5485 (2008-04-04)
Capsomeres are considered to be an alternative to viruslike particle (VLP)-based vaccines as they can be produced in prokaryotic expression systems. So far, no detailed side-by-side comparison of VLPs and capsomeres has been performed. In the present study, we immunized
Christina Schellenbacher et al.
Journal of virology, 83(19), 10085-10095 (2009-07-31)
The amino (N) terminus of the human papillomavirus (HPV) minor capsid protein L2 can induce low-titer, cross-neutralizing antibodies. The aim of this study was to improve immunogenicity of L2 peptides by surface display on highly ordered, self-assembled virus-like particles (VLP)
Avi-Hai Hovav et al.
Infection and immunity, 73(1), 250-257 (2004-12-25)
In this study, we examined the immunogenicity and protective efficacy of six immunodominant Mycobacterium tuberculosis recombinant antigens (85B, 38kDa, ESAT-6, CFP21, Mtb8.4, and 16kDa) in a multivalent vaccine preparation (6Ag). Gamma interferon (IFN-gamma) and monophosphoryl lipid A-trehalose dicorynomycolate (Ribi) adjuvant
Gabriel Ozorowski et al.
Journal of virology, 92(13) (2018-04-20)
Native-like, soluble, recombinant SOSIP trimers of various designs and based on several env genes of human immunodeficiency virus type 1 (HIV-1) are being tested as immunogens in different animal models. These experiments almost always involve coformulating the trimers with an
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Questions

1–8 of 8 Questions  

  1. Is the adjuvant ready to use? I don't see any mention in the datasheet on how to prepare the Antigen:adjuvant mixture. Can you help with that? Thank you.

    1 answer

    1. This product requires dilution with saline prior to use. This dilution can be done with or without the addition of the antigen. See below for a brief protocol.

      Prior to antigen addition, warm contents of vial to 40-45 °C. Add 2.0 mL of sterile saline containing the desired amount of antigen.
      The final emulsion will contain a concentration of 2% oil.
      1. Inject antigen-saline solution (2 mL) directly into the vial through the rubber stopper using a syringe fitted with a 20 or 21-gauge needle (leave the cap seal in place).
      2. Vortex the vial vigorously for 2 to 3 minutes to form emulsion. Invert the vial and vortex for 1 minute to ensure reconstitution of any product adhering to the stopper.
      3. If the entire contents of the vial will not be used initially, reconstitute with 1 ml saline without antigen. This emulsion can be stored at 2-8 °C for up to 60 days. Do Not freeze.
      To use, mix aliquots 1:1 with antigen in saline.
      4. Prior to inoculation, warm the vial to 37 °C and vortex briefly.

      For full details on injection protocols and boosting, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. When using the Sigma Adjuvant System® reagent, Product No. S6322, how do the components MPL and TDM work as an adjuvant?

    1 answer

    1. MPL (monophosphoryl lipid A) will bind to CD14 on the cell surface similar to LPS.  TDM (trehalose dicorynomycolate) is an analog of one of the components of the tubercle bacillus. Both of these components act like a superantigen and activate the immune system nonspecifically.  This allows for a greater response to the immunogen delivered in this adjuvant.

      Helpful?

  4. Can you reconstitute and store the Sigma Adjuvant System® reagent (Product No. S6322) if not using the entire vial right away?

    1 answer

    1. If the entire contents of the vial will not be used initially, reconstitute with 1 ml saline without antigen.  This emulsion can then be stored at 2-8 °C for up to 60 days.  Do Not Freeze. To use, mix aliquots 1:1 with antigen in saline. Prior to inoculation, warm the vial to 37 °C and vortex briefly.  This and other information can be found on the Sigma Adjuvant System® reagent (Product No. S6322) Product Information Sheet.

      Helpful?

  5. What is used to solubilize Product No. S6322, Sigma Adjuvant System® reagent?

    1 answer

    1. The Sigma Adjuvant System® reagent (Product No. S6322) can be solubilized in a sterile saline solution containing the desired amount of antigen to a final volume of 2 ml.  For more details, please see the S6322 Product Information Sheet.

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  6. Is the Sigma Adjuvant System®, Product No S6322, the same as the RIBI adjuvant?

    1 answer

    1. Yes, the Sigma Adjuvant System®, Product No S6322, is also called RIBI adjuvant.

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  7. Has Sigma Adjuvant System®, Product S6322, been used to induce EAE (Experimental autoimmune encephalomyelitis)?

    1 answer

    1. Sigma Adjuvant System®, Product S6322 is a replacement for MPT+TDM adjuvant (Ribi, Product M6536) that was discontinued by the supplier Corixa.  Although we have not tested Product S6322 for induction of Experimental autoimmune encephalomyelitis (EAE), the following reference cites the use of MBP in Ribi adjuvant for induction of acute EAE.The Journal of ImmunologyOctober 15, 2005, 175(8): 5077-5086

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  8. When using the Sigma Adjuvant System®, Product No. S6322, how is the antigenic response boosted?

    1 answer

    1. With the Sigma Adjuvant System® (Product No. S6322) with mice, rabbits, rats and guinea pigs; boost on day 21 or 28, bleed 10 - 14 days after booster injection. Boost every 3 - 4 weeks thereafter. For goats; boost every four weeks. Use the same protocol for each injection.  For more information please see the Product Information Sheet for Product No. S6322.

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