Saltar al contenido
Merck

P1484

Sigma-Aldrich

Anti-p21WAF1/Cip1antibody, Mouse monoclonal

clone CP74, purified from hybridoma cell culture

Sinónimos:

Anti-CDKN1A, Anti-Cip1, Anti-WAF1, Anti-p21

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización

Seleccione un Tamaño

0.2 ML
$669.000

$669.000


Fecha estimada de envío29 de abril de 2025

Está disponible un anticuerpo recombinante y sin conservantes para su diana. Pruebe ZRB1141

Solicitar un pedido a granel

Seleccione un Tamaño

Cambiar Vistas
0.2 ML
$669.000

About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

$669.000


Fecha estimada de envío29 de abril de 2025

Está disponible un anticuerpo recombinante y sin conservantes para su diana. Pruebe ZRB1141

Solicitar un pedido a granel

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

CP74, monoclonal

Formulario

buffered aqueous solution

mol peso

antigen 21 kDa

reactividad de especies

mouse, human

concentración

~1 mg/mL

técnicas

immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-10 μg/mL using LNCap cells (human prostate adenocarcinoma) treated with MG132 proteosome inhibition.

isotipo

IgG2b

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CDKN1A(1026)
mouse ... Cdkn1a(12575)

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 4

Este artículo
A4605C8218SAB1403659
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

mouse, human

species reactivity

mouse, human

species reactivity

rat, mouse, human

species reactivity

human

Gene Information

human ... CDKN1A(1026)
mouse ... Cdkn1a(12575)

Gene Information

human ... PPP1R13L(10848)
mouse ... Ppp1r13l(333654)

Gene Information

human ... CDK4(1019)
mouse ... Cdk4(12567)
rat ... Cdk4(94201)

Gene Information

human ... CDKN1B(1027)

clone

CP74, monoclonal

clone

LXO49.3, monoclonal

clone

DCS-31, monoclonal

clone

2F4, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Descripción general

Monoclonal Anti-p21WAF1/Cip1 (mouse IgG2b isotype) is derived from the CP74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant human p21WAF1/Cip1. p21WAF1/Cip1 is 21 kDa and designated as Cip1, WAF1, Sdi1, Pic1, CAP20.
Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody reacts specifically with p21WAF1/Cip1. Two families of CKIs have been identified. The p21WAF1/Cip1 family contains p21WAF1/Cip1, p27Kip1 and p57Kip2.

Inmunógeno

recombinant human p21WAF1/Cip1.

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody may be used for ELISA, immunoblotting (21 kDa) and immunoprecipitation (p21/cyclin A/Cdk2 complexes, native and denatured). The availability of a monoclonal antibody reacting specifically with p21WAF1/Cip1 enables the subcellular detection and localization of p21WAF1/Cip1 and the measurement of relative differences in p21WAF1/Cip1 levels as a function of cell cycle phase.

Acciones bioquímicas o fisiológicas

Regulation of cell cycle progression in eukaryotic cells depends on the expression of proteins called cyclins. These proteins form complexes with several different cyclin dependent kinases (CDKs). The catalytic activity of cyclin dependent kinases (CDK) is regulated by two general mechanisms: protein phosphorylation and association with regulatory subunits, which include the cyclins and the CDK inhibitors (CKIs). These inhibit all kinases involved in the G1/S transition. The the p16INK4a family, which includes p15INK4b, p16INK4a, p18INK4c and p19INK4d, inhibits Cdk4 and Cdk6 specifically.
The p21WAF1/Cip1 inhibit all kinases involved in the G1/S transition of cell cycle. p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is relatively a poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.

Forma física

Solution in phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Opcional

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Lu Deng et al.
Cell death & disease, 9(12), 1143-1143 (2018-11-18)
Myristoylation is one of key post-translational modifications that involved in signal transduction, cellular transformation and tumorigenesis. Increasing evidence demonstrates that targeting myristoylation might provide a new strategy for eliminating cancers. However, the underlying mechanisms are still yielded unclear. In this
Tumor susceptibility of p21Waf1/Cip1-deficient mice
Martin- Caballero J, et al.
Cancer research, 61(16), 6234-6238 (2001)
Isabel de Pedro et al.
Cell death & disease, 9(11), 1094-1094 (2018-10-27)
The epidermis is a self-renewal epithelium continuously exposed to the genotoxic effects of ultraviolet (UV) light, the main cause of skin cancer. Therefore, it needs robust self-protective mechanisms facing genomic damage. p53 has been shown to mediate apoptosis in sunburn
T Feng et al.
Oncogenesis, 6(1), e294-e294 (2017-01-31)
TGF-β signaling in liver cells has variant roles in the dynamics of liver diseases, including hepatocellular carcinoma (HCC). We previously found a correlation of high levels of the important endogenous negative TGF-β signaling regulator SMAD7 with better clinical outcome in
Yuji Wang et al.
The Journal of biological chemistry, 287(31), 25941-25953 (2012-05-19)
Tumor suppressor genes are frequently silenced in cancer cells by enzymes catalyzing epigenetic histone modifications. The peptidylarginine deiminase family member PAD4 (also called PADI4) is markedly overexpressed in a majority of human cancers, suggesting that PAD4 is a putative target

Questions

Reviews

No rating value

Active Filters

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico