Saltar al contenido
Merck

Documentos clave

Ver Toda la documentación

Saltar a

B5002

5-Bromo-2′-desoxiuridina

≥99% (HPLC), synthetic (organic), powder

Sinónimos:

5- BrdU, 5-Bromo-1-(2-desoxi-β-D-ribofuranosil)uracilo, BUdR, Desoxirribósido de 5-bromouracilo

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
A ustedes/SKUDisponibilidadPrecio
100 mg
Comprobar disponibilidad del carrito
$66.400
250 mg
Comprobar disponibilidad del carrito
$83.800
500 mg
Comprobar disponibilidad del carrito
$127.000
1 g
Comprobar disponibilidad del carrito
$225.000
5 g
Comprobar disponibilidad del carrito
$622.000
100 g
Comprobar disponibilidad del carrito
$9.190.000

Acerca de este artículo

Fórmula empírica (notación de Hill):
C9H11BrN2O5
Número CAS:
59-14-3
Peso molecular:
307.10
NACRES:
NA.51
PubChem Substance ID:
24278270
UNSPSC Code:
41106305
EC Number:
200-415-9
MDL number:
MFCD00006529
Beilstein/REAXYS Number:
30395
Assay:
≥99% (HPLC)
Biological source:
synthetic (organic)
Form:
powder
Solubility:
DMSO: 50 mg/mL, clear, colorless to very faintly yellow
Storage temp.:
−20°C

$66.400

Comprobar disponibilidad del carrito
​
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle

Nombre del producto

5-Bromo-2′-desoxiuridina, ≥99% (HPLC)

biological source

synthetic (organic)

Quality Segment

200

assay

≥99% (HPLC)

form

powder

mp

191-194 °C (dec.) (lit.)

solubility

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI key

WOVKYSAHUYNSMH-RRKCRQDMSA-N

Application

La 5-bromo-2′-desoxiuridina (5-BrdU) es un análogo de la timidina que se incorpora al ADN. La 5-BrdU se utiliza habitual y extensamente para medir la síntesis de ADN y marcar las células en división. Por consiguiente, la 5-BrdU se utiliza para estudiar la comunicación celular y otros procesos que inducen proliferación celular.
La 5-bromo-2′-desoxiuridina se ha utilizado:
  • como suplemento para estudiar el efecto de las crisis electroconvulsivas en la neurogénesis del hipocampo
  • como suplemento para estudiar la pérdida de función génica en el corazón del pez cebra adulto
  • en el ensayo de proliferación de linfocitos T{133]

Biochem/physiol Actions

Análogo de timidina utilizado como mutágeno en investigación genética. Incorporada de manera selectiva en el ADN celular durante la fase S.
La 5-bromo-2′-desoxiuridina (BrdU) se utiliza para analizar la proliferación celular, por su fácil incorporación al ADN durante la fase S del ciclo celular. La BrdU estimula la diferenciación y la maduración celular en las líneas celulares leucémicas, mientras que inhibe la diferenciación de las células eritroleucémicas amigas. En los estudios de incorporación de la BrdU, a menudo se utilizan después anticuerpos específicos de la BrdU con etiquetas fluorescentes para la detección de la BrdU incorporada, mediante métodos como la citometría de flujo y la microscopia de fluorescencia.

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
B9285D5412I7125
5-Bromo-2′-desoxiuridina ≥99% (HPLC)

Sigma-Aldrich

B5002

5-Bromo-2′-desoxiuridina

5-Bromo-2′-desoxiuridina BioUltra, ≥99%

Sigma-Aldrich

B9285

5-Bromo-2′-desoxiuridina

2′-Deoxyuridine ≥98.5%

Sigma-Aldrich

D5412

2′-Deoxyuridine

5-Iodo-2′-deoxyuridine ≥99% (HPLC)

Sigma-Aldrich

I7125

5-Iodo-2′-deoxyuridine

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

synthetic (organic)

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

assay

≥99% (HPLC)

assay

≥99%

assay

≥98.5%

assay

≥99% (HPLC)

form

powder

form

powder

form

powder

form

powder or crystals

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

2-8°C

solubility

DMSO: 50 mg/mL, clear, colorless to very faintly yellow

solubility

NH4OH: 0.1 M at 20 °C, clear, colorless

solubility

water: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

solubility

1 N NH4OH: 50 mg/mL, clear to very slightly hazy, colorless to light yellow

Still not finding the right product?

Explore all of our products under 5-Bromo-2′-desoxiuridina

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Muta. 1B - Repr. 2

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Questions

1–8 of 8 Questions  

  1. Could specific insights into the use of this product be provided? BrdU was administered in water to mice for up to 2 weeks for an in vivo cell proliferation assay. However, upon staining with an anti-BrdU antibody, no BrdU incorporation was detected in the positive control (small intestine), despite trying various concentrations. Any information on the particular use of this product would be much appreciated

    1 answer

    1. Given the BrdU incorporation into DNA of cells, using the Western blot method for detection would be quite challenging. Typically, the immunohistochemistry method is employed for this purpose. When it comes to the application on small intestine samples, the following citation of B5002 via immunohistochemical detection may be useful for protocol reference:

      Title: "The ubiquitin ligase ITCH coordinates small intestinal epithelial homeostasis by modulating cell proliferation, differentiation, and migration." Published in Differentiation. Jan-Feb 2018;99:51-61. PMID: 29309986

      Helpful?

  2. How long can BrdU be stored at -20 degrees and -4 degrees, after being diluted in sterile saline and being filtered with a 0.22 µm filter?

    1 answer

    1. BrdU should be stored at –20 oC and desiccated. Stock solutions can be made in DMSO to up to 50 mg/mL, as indicated in the Product information sheet. The stock can be stored for 6 months at -20°C in single use aliquots.

      No information is available on storage conditions for BrdU dissolved in saline.

      Helpful?

  3. What is the stability of Product B5002, BrdU?

    1 answer

    1. BrdU is stable as a powder, kept in the freezer and has a recommended re-test date of two years according to the Product Specification Sheet for Product No. B5002.  Please consult our Product Dating Information Statement for more information on re-test dated products.

      Helpful?

  4. How long do I treat cells with Product B5002, BrdU to incorporate it into the DNA?

    1 answer

    1. Actively growing cells may be treated with 10 μm BrdU for 1 hour.

      Helpful?

  5. What are the hazards associated with BrdU, Product B5002?

    1 answer

    1. BrdU is a known carcinogen and caution should be exercised when handling.  Click on the MSDS hyperlink located on the product detail page for Product No. B5002, 5-Bromo-2´-deoxyuridine.

      Helpful?

  6. What can I use to detect BrdU, Product B5002 in vivo and in vitro?

    1 answer

    1. For the immunohistochemical detection of BrdU, we recommend Product No. B2531, Monoclonal Anti-BrdU antibody produced in mouse.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. How does BrdU, Product B5002, work as a Thymidine analog?

    1 answer

    1. BrdU replaces Thymidine during the S-phase of cell division.

      Helpful?

Reviews

No rating value

Active Filters