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MDQ1

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Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

Sinónimos:

DNA Methylation Quantification, DNA methylation shift

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.54

Descripción general

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Aplicación

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Características y beneficios

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Información legal

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • M8570Methylated Control DNA, 50 ng/μLSDS

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Pictogramas

CorrosionExclamation mark
GHS05,GHS07

Palabra de señalización

Warning

Frases de peligro

H290,H317,H412

Clasificaciones de peligro

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

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Certificados de análisis (COA)

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Roman Thaler et al.
The British journal of nutrition, 101(5), 743-749 (2008-08-08)
The impact of nutrition on the epigenetic machinery has increasingly attracted interest. The aim of the present study was to demonstrate the effects of various diets on methylation and gene expression. The antioxidative enzyme mitochondrial superoxide dismutase (MnSOD) was chosen
Svetlana I Novikova et al.
PloS one, 3(4), e1919-e1919 (2008-04-03)
Previous studies documented significant behavioral changes in the offspring of cocaine-exposed mothers. We now explore the hypothesis that maternal cocaine exposure could alter the fetal epigenetic machinery sufficiently to cause lasting neurochemical and functional changes in the offspring. Pregnant CD1
Felicitas Thol et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 29(21), 2889-2896 (2011-06-15)
To study the incidence and prognostic impact of mutations in DNA methyltransferase 3A (DNMT3A) in patients with acute myeloid leukemia. A total of 489 patients with AML were examined for mutations in DNMT3A by direct sequencing. The prognostic impact of
Mounira Krifa et al.
Journal of experimental & clinical cancer research : CR, 32, 30-30 (2013-05-22)
Several reports have described the potential effects of natural compounds as anti-cancer agents in vitro as well as in vivo. The aim of this study was to evaluate the anti-cancer effect of Limoniastrum guyonianum aqueous gall extract (G extract) and
Global DNA methylation profile in medullary thyroid cancer patients
Ceolin L, et al.
Experimental and Molecular Pathology (2018)
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Protocolos

Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol

The Imprint® Methylated DNA Quantification Kit provides a high-throughput, non-radioactive means of measuring global DNA methylation shifts.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

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