If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
MAK134
Ketone Body Assay
sufficient for 200 tests (UV)
Sinónimos:
Ketone Quantification Kit
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About This Item
Productos recomendados
uso
sufficient for 200 tests (UV)
Características
This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.
método de detección
colorimetric
enfermedades relevantes
endocrinological disorders, diabetes; neonatal diseases; pediatric diseases; cancer
temp. de almacenamiento
−20°C
Categorías relacionadas
Descripción general
Aplicación
Características y beneficios
Idoneidad
Principio
Código de clase de almacenamiento
10 - Combustible liquids
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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is this kit compatible for mouse serum/plasma?
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Yes, this kit have been used successfully in mouse, rat, bovine, and human samples.
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What is the source of the HBDH enzyme?
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The species and source of the HBDH enzyme (3-hydroxybutyrate dehydrogenase) in the assay is native Acaligenes faecalis.
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What is the sensitivity range of this kit?
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The kit is sensitive and accurate, utilizing a 10 μL sample. It offers a linear detection range of 0.12 to 8 mM for each ketone body in a 96-well plate assay.
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Is whole blood or plasma collected in EDTA suitable for use with MAK134 Ketone Body Assay?
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Certainly, whole blood or plasma collected in EDTA is appropriate for use with this kit. During the development of the kit, these sample types were tested as they are commonly used by researchers. And also AcAc (Acetoacetic acid) is quite unstable, so it is advised using fresh samples or ones that have been frozen within the month.
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What is the range of quantification for both analytes in this assay kit?
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This kit has a linear detection range of 0.12 to 8 mM for each ketone body in a 96-well plate assay.
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