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MAK134

Sigma-Aldrich

Ketone Body Assay

sufficient for 200 tests (UV)

Sinónimos:

Ketone Quantification Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 200 tests (UV)

Características

This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.

método de detección

colorimetric

enfermedades relevantes

endocrinological disorders, diabetes; neonatal diseases; pediatric diseases; cancer

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Ketone bodies, 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc), are produced in the liver primarily from oxidation of fatty acids, and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypoglycemia, growth hormone deficiency, alcohol or salicylate intoxication, and inborn errors of metabolism.

Aplicación

Ketone Body Assay has been used to measure the release of ketone bodies in the human liver cancer cell line HepG2 culture medium.

Características y beneficios

Compatible with high-throughput handling systems

Idoneidad

Suitable for the detection of ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) in serum, plasma, urine, and other biological samples.

Principio

In this kit, AcAc and BOH levels are determined using an enzymatic assay based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance measured at 340 nm is directly related to either the AcAc and BOH concentrations.

Código de clase de almacenamiento

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
134CF01A08
134CF01A08
134CE06A28
134CE06A28
134CD11A21

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Role of β-hydroxybutyrate, its polymer poly-β-hydroxybutyrate and inorganic polyphosphate in mammalian health and disease.
Dedkova E N and Lothar A B
Frontiers in Physiology, 5, 260-260 (2014)
Zachary M Augur et al.
Frontiers in nutrition, 5, 91-91 (2018-10-24)
Introduction: Temozolomide (TMZ) is part of the standard of care for treating glioblastoma multiforme (GBM), an aggressive primary brain tumor. New approaches are needed to enhance therapeutic efficacy and reduce toxicity. GBM tumor cells are dependent on glucose and glutamine
Hyperketonemia and ketosis increase the risk of complications in type 1 diabetes.
Kanikarla M, et al.
Free Radical Biology & Medicine, 95, 268-277 (2016)
Kim J Shimy et al.
Journal of the Endocrine Society, 4(7), bvaa062-bvaa062 (2020-07-16)
According to the carbohydrate-insulin model of obesity, an elevated insulin-to-glucagon ratio in response to a high-carbohydrate diet directs metabolic fuels toward storage, resulting in lower circulating energy. To determine differences in total circulating energy post-meal related to dietary carbohydrate. Ancillary
Catia Martins et al.
Obesity (Silver Spring, Md.), 28(12), 2331-2338 (2020-11-25)
The purpose of this study was to examine whether the degree of ketosis, measured as plasma β-hydroxybutyrate (βHB) in fasting, was associated with changes in appetite feelings and plasma concentration of appetite-related hormones after weight loss. A total of 87
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