M9281

3-Methyladenine

autophagy inhibitor

• Sinónimos: 3-MA, 6-Amino-3-methylpurine
• Fórmula empírica (notación de Hill): C₆H₇N₅
• Número de CAS: 5142-23-4
• Peso molecular: 149.15
• Beilstein: 146087
• Número CE: 225-908-6
• Número MDL: MFCD00010531
• Código UNSPSC: 41106305
• ID de la sustancia en PubChem: 24897326
• NACRES: NA.51

Propiedades

• origen biológico: synthetic (organic)
• Nivel de calidad: 300
• Ensayo: ≥99% (HPLC)
• Formulario: powder
• mp: ~300 °C (dec.) (lit.)
• solubilidad: DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow
• cadena SMILES: Cn1cnc(N)c2ncnc12
• InChI: 1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3
• Clave InChI: FSASIHFSFGAIJM-UHFFFAOYSA-N

Descripción

Aplicación

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.

3-Methyladenine has been used as an autophagy inhibitor:

• to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs)
• with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells
• to study the interplay between colistin-induced autophagy and apoptosis

Standard for detection of nucleic acid methylation during carcinogenesis.

Acciones bioquímicas o fisiológicas

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).

Nota de preparación

This product is soluble in DMF (10 mg/mL; may require gentle heating). It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/mL with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA may be prepared in DMSO at 100 mM.

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable