H8283
Hemocyanin from Megathura crenulata (keyhole limpet)
PBS solution
Sinónimos:
KLH, Keyhole limpet hemocyanin
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About This Item
Productos recomendados
biological source
Megathura crenulata
Quality Level
form
PBS solution
concentration
3.0-7.0 mg/mL protein (A280)
copper content
0.10-0.30% (based on protein)
technique(s)
ELISA: suitable
storage temp.
2-8°C
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General description
Gastropod hemocyanins are massive glycoproteins (4 to 8 MDa) designed by an intricate arrangement of 10 subunits that are self-assembled into hollow cylinders 35 nm in diameter.
Hemocyanin is a high molecular weight copper containing glycoprotein. It reversibly binds oxygen and forms the extra-cellular respiratory protein of mollusks. Keyhole limpet hemocyanin (KLH) is filtered from the hemolymph of Megathura crenulata, also called the giant keyhole limpet. It is native to the southern California coast and Mexico.
Application
Keyhole limpet hemocyanin (KLH) is a strong and well known immune-stimulant in both experimental animals and humans. It has been used as a hapten carrier protein to enhance antigen-specific T cell priming and is known to stimulate a CD4+ T-cell response.
Hemocyanin was used to study if antipeptide antibodies specifically recognize the l1 protein of the human papilloma virus of type 31. It was also used to study the acid-induced unfolding of didecameric keyhole limpet hemocyanin.
Hemocyanin was used to study if antipeptide antibodies specifically recognize the l1 protein of the human papilloma virus of type 31. It was also used to study the acid-induced unfolding of didecameric keyhole limpet hemocyanin.
Physical form
Solution in PBS, pH 7.2, containing 1mM CaCl2 and 0.5 mM MgCl2 with 15 mM sodium azide as preservative
Preparation Note
Chromatographically purified.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Hendrikus J Wijnen et al.
Frontiers in veterinary science, 8, 784869-784869 (2022-01-01)
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Amino acids, 39(3), 899-910 (2010-03-10)
Keyhole limpet hemocyanin (KLH) is widely used as an immune stimulant and hapten carrier derived from a marine mollusc Megathura crenulata. To provide details of the stability and equilibrium of KLH, different intermediate species were investigated with a series of
S M Andreev et al.
Bioorganicheskaia khimiia, 38(6), 667-675 (2013-04-04)
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The tannins, delphinidin and procyanidin were isolated from flowers of white clover (Trifolium repens) and the leaves of Arnot Bristly Locust (Robina fertilis) respectively, and tested for mutagenic properties in a range of systems. There was no evidence for either
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