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Merck

F6636

SAFC

Nutrient Mixture F-12 Ham

Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Ham’s F-12

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

Nombre del producto

Nutrient Mixture F-12 Ham, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification, suitable for cell culture

Formulario

powder

técnicas

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

componentes

phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Aplicación

Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.

Cantidad

Formulated to contain 11.5 grams of powder per liter of medium.

Reconstitución

Supplement with 2.68 g/L sodium bicarbonate.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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In-frame premature termination codons (PTCs) account for ∼11% of all disease-associated mutations. PTC suppression therapy utilizes small molecules that suppress translation termination at a PTC to restore synthesis of a full-length protein. PTC suppression is mediated by the base pairing
Feng Liang et al.
SLAS technology, 22(3), 315-324 (2017-04-06)
Cystic fibrosis (CF) is a hereditary disease caused by mutations in the gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR). A large number of nearly 2000 reported mutations, including the premature termination codon (PTC) mutations, urgently require new
Kenneth A Giuliano et al.
SLAS discovery : advancing life sciences R & D, 23(2), 111-121 (2017-09-13)
Cystic fibrosis (CF) is a lethal genetic disorder caused by mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Despite recent groundbreaking approval of genotype-specific small-molecule drugs, a significant portion of CF patients still lack effective therapeutic options that
Wren E Michaels et al.
Nucleic acids research, 48(13), 7454-7467 (2020-06-11)
Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, encoding an anion channel that conducts chloride and bicarbonate across epithelial membranes. Mutations that disrupt pre-mRNA splicing occur in >15% of
Nyla Naim et al.
Methods in molecular biology (Clifton, N.J.), 2173, 201-216 (2020-07-12)
cAMP is a crucial mediator of multiple cell signaling pathways. This cyclic nucleotide requires strict spatiotemporal control for effective function. Light-activated proteins have become a powerful tool to study signaling kinetics due to having quick on/off rates and minimal off-target

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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