F4024
Anti-Biotin–FITC antibody, Mouse monoclonal
clone BN-34, purified from hybridoma cell culture
Sinónimos:
Monoclonal Anti-Biotin
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0.2 ML
$328.000
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0.2 ML
$328.000
About This Item
Código UNSPSC:
12352203
NACRES:
NA.46
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
FITC conjugate
forma del anticuerpo
purified from hybridoma cell culture
tipo de anticuerpo
primary antibodies
clon
BN-34, monoclonal
Formulario
buffered aqueous solution
condiciones de almacenamiento
protect from light
técnicas
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80
isotipo
IgG1
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is an essential vitamin required by cells in living organisms or in cultures. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Inmunógeno
Biotin-KLH conjugate
Aplicación
May be substituted for avidin to decrease non-specific binding.
Monoclonal Anti-Biotin-FITC antibody produced in mouse has been used in the localization of the antigen with a primary antibody, addition of a biotinylated antibody to bind to primary antibody and finally application avidin-FITC.
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Acciones bioquímicas o fisiológicas
An immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Forma física
Solution in phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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