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Merck

F4024

Sigma-Aldrich

Anti-Biotin–FITC antibody, Mouse monoclonal

clone BN-34, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-Biotin

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0.2 ML
$328.000

$328.000


Fecha estimada de envío03 de mayo de 2025


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0.2 ML
$328.000

About This Item

Código UNSPSC:
12352203
NACRES:
NA.46

$328.000


Fecha estimada de envío03 de mayo de 2025


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origen biológico

mouse

Nivel de calidad

conjugado

FITC conjugate

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

BN-34, monoclonal

Formulario

buffered aqueous solution

condiciones de almacenamiento

protect from light

técnicas

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is an essential vitamin required by cells in living organisms or in cultures. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Inmunógeno

Biotin-KLH conjugate

Aplicación

May be substituted for avidin to decrease non-specific binding.
Monoclonal Anti-Biotin-FITC antibody produced in mouse has been used in the localization of the antigen with a primary antibody, addition of a biotinylated antibody to bind to primary antibody and finally application avidin-FITC.
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Acciones bioquímicas o fisiológicas

An immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Forma física

Solution in phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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