E3763

Resorufin ethyl ether

≥98% (TLC), powder

• Sinónimos: 7-Ethoxy-3H-phenoxazin-3-one, Ethoxyresorufin, O⁷-Ethylresorufin
• Fórmula empírica (notación de Hill): C₁₄H₁₁NO₃
• Número de CAS: 5725-91-7
• Peso molecular: 241.24
• Beilstein: 225973
• Número MDL: MFCD00037661
• Código UNSPSC: 12161501
• ID de la sustancia en PubChem: 24894527
• NACRES: NA.47

Propiedades

• Nivel de calidad: 200
• Ensayo: ≥98% (TLC)
• Formulario: powder
• condiciones de almacenamiento: (Tightly closed. Dry)
• técnicas: activity assay: suitable
• color: orange to red
• mp: 223-225 °C (lit.)
• solubilidad: chloroform: 9.80-10.20 mg/mL, clear, orange
• idoneidad: suitable for fluorescence
• temp. de almacenamiento: −20°C
• cadena SMILES: CCOc1ccc2N=C3C=CC(=O)C=C3Oc2c1.Fc4c(F)c(F)c(OC(=O)CNC(=O)OCC5c6ccccc6-c7ccccc57)c(F)c4F
• InChI: 1S/C23H14F5NO4.C14H11NO3/c24-17-18(25)20(27)22(21(28)19(17)26)33-16(30)9-29-23(31)32-10-15-13-7-3-1-5-11(13)12-6-2-4-8-14(12)15;1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h1-8,15H,9-10H2,(H,29,31);3-8H,2H2,1H3
• Clave InChI: ZOSYTBPPLWBBKM-UHFFFAOYSA-N

Descripción

Descripción general

Resorufin ethers are used as markers or substrates for different cytochrome P (CYP) isoforms. Resorufin ethyl ether is the preferred resorufin ether to be metabolized by microsomes of 3-methylcholanthrene treated animals.

Resorufin is the reduced product of resazurin, also termed as Alamar Blu. It exhibits fluorescence at longer wavelengths and is flexible for modification in its hydroxyl group.

Research area: Cell Signaling

Aplicación

Resorufin ethyl ether is suitable for the study of dealkylating activity of cytochrome P450 isozymes 1A1 and 1A2, where arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and A′-acetylcysteine conjugates are used as inhibitors. It is suitable to study the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 activity (CYP1A1).

Sustratos

Fluorimetric substrate for cytochrome P450 linked enzyme, CXPIA1/2

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)