LA-4

90040512, mouse lung, Epithelial-like

• UNSPSC Code: 41106514

Propiedades

• product name: LA-4, 90040512
• biological source: mouse lung
• growth mode: Adherent
• karyotype: Unstable, range 38-256 chromosomes
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse A/He lung adenoma

Cell Line Description

Isolated from a urethan-induced lung adenoma of a 28 week old A/He strain mouse. The cells incorporate high levels of 14C-choline into lecithin. C-type particles have been observed in some cultures.

Application

Tumourigenicity studies

Culture Medium

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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