C4519

Ceruloplasmin human

lyophilized powder, oxidase 20-75 units/mg protein

• Número de CAS: 9031-37-2
• Número MDL: MFCD00130766
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Formulario: lyophilized powder
• Nivel de calidad: 200
• oxidase activity: 20-75 units/mg protein
• mol peso: ~135 kDa
• composición: Protein, ~30%
• contenido de cobre: 1.0-2.5 μg/mg protein
• Nº de acceso UniProt: P00450
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... CP(1356)

Descripción

Acciones bioquímicas o fisiológicas

Ceruloplasmin (Cp), the principal carrier of copper in plasma, is thought to be involved in oxidative modification of lipoproteins and participate in the acute phase reaction to stress. Cp is an effective antioxidant for a variety of radicals and has a potent peroxidase activity to decompose hydrogen peroxide in the presence of reduced glutathione. It inhibits the peroxidase activity of myeloperoxidase in a concentration-dependent manner and shows selective binding to myeloperoxidase in vitro. This suggests that ceruloplasmin may participate in the clearance and inactivation of myeloperoxidase in vivo. At physiological (micromolar) concentrations it was found to inhibit relaxation of rabbit aorta induced by endothelium-dependent agonists like acetylcholine or ADP. However, it was found ineffective toward vasodilation due to direct stimulation of smooth muscle cells by nitroglycerin.

Ceruloplasmin (Cp), the principal carrier of copper in plasma, is thought to be involved in oxidative modification of lipoproteins and participate in the acute phase reaction to stress. Cp is an effective antioxidant for a variety of radicals and has a potent peroxidase activity to decompose hydrogen peroxide in the presence of reduced glutathione. It inhibits the peroxidase activity of myeloperoxidase in a concentration-dependent manner and shows selective binding to myeloperoxidase in vitro. This suggests that ceruloplasmin may participate in the clearance and inactivation of myeloperoxidase in vivo. At physiological (micromolar) concentrations it was found to inhibit relaxation of rabbit aorta induced by endothelium-dependent agonists like acetylcholine or ADP. However, it was found ineffective toward vasodilation due to direct stimulation of smooth muscle cells by nitroglycerin. Cp plays an essential role in iron homeostasis, regulating the efflux of iron from macrophages and the oxidation of Fe²⁺ to Fe³⁺. Cp deficiency leads to neurodegenerative disorders, possibly by starving neurons of iron.

Definición de unidad

One unit will cause a ΔA₅₅₀ of 0.01 per min using N,N-dimethyl-p-phenylenediamine as substrate at pH 5.5 and 37 °C, in a 7 mL reaction volume.

Forma física

Pale blue powder containing sodium chloride and sodium acetate

Nota de análisis

Protein determined by biuret

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable