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30915

Sigma-Aldrich

Denhardt′s solution

BioReagent, for molecular biology, lyophilized powder

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About This Item

MDL number:
MFCD00717549
UNSPSC Code:
12352202
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

form

lyophilized powder

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

suitability

suitable for nucleic acid hybridization

storage temp.

2-8°C

General description

Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Application

Suitable to block non-specific binding sites in western, northern and Southern blot membranes

Components

Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.

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Referencia del producto
Descripción
Precios

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Disolución salina tamponada con fosfato, 10× PBS for Western blots and IP

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

H7140

Hybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

H7033

PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

D9905

Denhardt′s Solution, for Northern and Southern blotting, lyophilized powder

D2532

Denhardt′s Solution 50x, for Northern and Southern blotting, solution

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

C7594

Casein Blocking Buffer, for Northern and Southern blotting, powder blend

93017

SSC Buffer 20x, for Northern and Southern blotting, solution

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Flavien Pillet et al.
Nucleic acids research, 39(17), 7477-7486 (2011-06-10)
The segregation of plasmid F of Escherichia coli is highly reliable. The Sop partition locus, responsible for this stable maintenance, is composed of two genes, sopA and sopB and a centromere, sopC, consisting of 12 direct repeats of 43 bp. Each
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual (1989)
Ren Li et al.
Nature communications, 11(1), 5844-5844 (2020-11-19)
Fruit firmness is a target trait in tomato breeding because it facilitates transportation and storage. However, it is also a complex trait and uncovering the molecular genetic mechanisms controlling fruit firmness has proven challenging. Here, we report the map-based cloning

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