B3928
Blue-White Select™ Screening Reagent
for selection of recombinant bacterial clones
Sinónimos:
Blue-White Reagent, Screening Reagent, Selection Reagent
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NACRES:
NA.85
UNSPSC Code:
12352200
Servicio técnico
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Molecular Biology
Quality Segment
form
liquid
usage
sufficient for 125 dishes (90 mm)
technique(s)
nucleic acid detection: suitable
suitability
suitable for nucleic acid staining
shipped in
dry ice
storage temp.
−20°C
General description
Sigma′s Blue-White Select™ Screening Reagent is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Blue-White Select™ Screening Reagent is designed for blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Biochem/physiol Actions
When Blue-White Select™ Screening Reagent is cleaved by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Blue-White Select™ Screening Reagent remain white in color, allowing for easy differentiation between lac+ and lac- colonies.
The technique is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an a-fragment of β-galactosidase. Exploitation of these vectors requires use of a bacteria strain carrying the complementing gene fragment to allow the assembly of an active complex, resulting in the formation of blue colonies. Disruption of the β-galactosidase gene by insertion of a DNA fragment into the vector′s multiple cloning site results in the loss of functional β-galactosidase activity; these colonies remain white, allowing for easy differentiation between lac+ and lac- colonies.
The technique is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an a-fragment of β-galactosidase. Exploitation of these vectors requires use of a bacteria strain carrying the complementing gene fragment to allow the assembly of an active complex, resulting in the formation of blue colonies. Disruption of the β-galactosidase gene by insertion of a DNA fragment into the vector′s multiple cloning site results in the loss of functional β-galactosidase activity; these colonies remain white, allowing for easy differentiation between lac+ and lac- colonies.
Features and Benefits
- Intense color contrast for easy colony selection
- Convenient, ready-to-use solution
Other Notes
40 mg/mL IPTG
40 mg/mL X-Gal
(prepared in DMSO)
40 mg/mL X-Gal
(prepared in DMSO)
Legal Information
Blue-White Select is a trademark of Sigma-Aldrich Co. LLC
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F
flash_point_c
87 °C
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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