11373242910

Fluorescein-12-dUTP

≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution

• Sinónimos: Fluorescein-12-dUTP, dUTP
• Código UNSPSC: 41116100

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥85% (HPLC)
• Formulario: solution
• mol peso: M_r 1018.4 (fluorescein-12-dUTP-Li₄)
• envase: pkg of 25 μL (25 nmol; 1mM)
• fabricante / nombre comercial: Roche
• técnicas: ELISA: suitable; hybridization: suitable
• temp. de almacenamiento: −20°C

Descripción

Aplicación

Fluorescein-12-dUTP is used as a substrate for:

• terminal transferase
• DNA polymerase I (holoenzyme and Klenow fragment),
• Taq DNA polymerase
• reverse transcriptase (e.g., from AMV and M-MuLV).

Fluorescein-12-dUTP has been used to label nuclear extracts during PCR.[1] It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL.[2] Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection[3] or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

Calidad

Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).

Forma física

Fluorescein-12-dUTP, tetralithium salt.

Almacenamiento y estabilidad

The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash