Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

View All Documentation

11207750910

Roche

Anti-Digoxigenin-Rhodamine, Fab fragments

from sheep

Sinónimos:

anti-digoxigenin, digoxigenin

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12352203

biological source

sheep

Quality Level

100

conjugate

rhodamine conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized (clear, red solution after reconstitution)

packaging

pkg of 200 μg

manufacturer/tradename

Roche

isotype

IgG

storage temp.

2-8°C

Categorías relacionadas

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to rhodamine.

Specificity

Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).

Application

Use Anti-Digoxigenin-Rhodamine, Fab fragments for the detection of digoxigenin-labeled compounds using:
  • Digoxigenin-labeled sugars in glycoconjugate research
  • Fluorescent in situ hybridization (FISH)
  • Immunohistocytochemistry
  • In situ hybridization
  • DNA fiber assay

Preparation Note

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
  • Fluorescent in situ hybridization (FISH): 1 to 20 μg/ml
  • Immunohistocytochemistry: 20 to 50 μg/ml
  • In situ hybridization: 20 to 50 μg/ml

Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.

Storage conditions (working solution): Always prepare fresh!

Reconstitution

Add 1 ml double-distilled water to a final concentration of 200 μg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Yansen Ma et al.
FEBS letters, 587(10), 1489-1495 (2013-03-29)
One of the major challenges in stem cell research is to decipher the controlling mechanisms/genes of stem cell homeostasis. Through an RNAi mediated genetic screen of living animals, we identified an evolutionarily conserved histone acetyltransferase Atac2 as a novel regulator
Spencer W Luebben et al.
Nucleic acids research, 41(22), 10283-10297 (2013-09-06)
HELQ is a superfamily 2 DNA helicase found in archaea and metazoans. It has been implicated in processing stalled replication forks and in repairing DNA double-strand breaks and inter-strand crosslinks. Though previous studies have suggested the possibility that HELQ is
Galina Pendinen et al.
Genome, 51(9), 714-720 (2008-09-06)
Thirty-six percent of the wild potato (Solanum L. section Petota Dumort.) species are polyploid, and about half of the polyploids are tetraploid species (2n = 4x = 48). Determination of the type of polyploidy and development of the genome concept
R Symonová et al.
Cytogenetic and genome research, 141(2-3), 153-162 (2013-09-21)
We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (∼120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (∼240 chromosomes) and a spontaneous triploid Siberian
Maximilian H Fitz-James et al.
eLife, 9 (2020-09-12)
During mitosis chromosomes reorganise into highly compact, rod-shaped forms, thought to consist of consecutive chromatin loops around a central protein scaffold. Condensin complexes are involved in chromatin compaction, but the contribution of other chromatin proteins, DNA sequence and histone modifications
Ver todos los artículos relacionados

Artículos

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico