Product TMS-010 is a standard pH 2.5 solution of Alcian Blue that stains a number of substances, including chondrocytes, at this pH. The provided procedure is similar but not identical to the standard histology stain procedure found in most histology textbooks.
TMS-010
Alcian-Blue Staining Solution
Sinónimos:
Alcian blue 8G, Alcian blue PAS stain, Alcian blue stain
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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técnicas
cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable
Nivel de calidad
Categorías relacionadas
Descripción general
Alcian blues, are a family of polyvalent basic dyes, of which, the Alcian blue 8G has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. Alcian blue if often used to identify Chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells.
Aplicación
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Osteobiology
Arthritis
Osteobiology
Arthritis
Calidad
Solution is blue with no particulates present. Solution is suitable for endpoint cell culture assays.
Forma física
Product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
Product is presented as a 1% w/v Alcian Blue solution.
Almacenamiento y estabilidad
Store at room temperature for up to 4 months from date of receipt. If Alcian-Blue solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A
Código de clase de almacenamiento
3 - Flammable liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
102.2 °F - closed cup
Punto de inflamabilidad (°C)
39 °C - closed cup
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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What is the composition of the differentiation for TMS 010 C procedure?
1 answer-
Helpful?
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What is the concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product?
1 answer-
The concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product is not provided. However, it is a classical textbook formulation typically consisting of 1% Alcian Blue in 3% glacial acetic acid.
Helpful?
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Is there a procedure available for using TMS-010, and in this context, would you anticipate the alcian blue to be present in the nuclei, cytoplasm, or the entire cell?
1 answer-
Depending on the preparation of Alcian Blue, the dye can be used to identify various substances. TMS-010 seems to have been formulated to stain chondrocytes, the cells that produce collagen. It's expected that the staining should be primarily in the cytoplasm. However, if the staining intensity is high, it might appear that the entire cell is stained.
Helpful?
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Can you provide a protocol for a colorimetric assay that uses Alcian Blue to compare sulfated proteoglycan content in adherent cancer cell lines?
1 answer-
The Alcian-Blue Staining Solution (cat. no. TMS-010-C) is a cell culture product from the cell culture portfolio, intended for cell-based assays, cell differentiation, and stem cell culture. For example, to identify chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells (MSCs).
This product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
The Certificate of Analysis does not include any usage instructions and there is no separate product data sheet or user guide.
The following paper may be helpful for the staining of stem cells:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432525/Helpful?
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