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Key Documents

GR08L

Sigma-Aldrich

Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14)

lyophilized, clone T56/14, Calbiochem®

Sinónimos:

Anti-CD71, Anti-TR, Anti-TR, Anti-CD71

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

T56/14, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TFRC(7037)

General description

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with S194/5XXO.BU.1 mouse myeloma cells (see application references). Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor protein.
This Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) is validated for use in FC, IF, IP, Immunoblotting for the detection of Transferrin Receptor (Ab-1).

Immunogen

K562 cells

Application

Flow Cytometry (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Immunoblotting (not recommended)

Warning

Toxicity: Standard Handling (A)

Reconstitution

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.

Other Notes

Schneider, C., et al., 1982. J. Biol. Chem.257, 8516.
Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem.49, 357.
Omary, M.B., et al. 1980. Nature286, 888.
Seligman, P.A., et al. 1979. J. Biol. Chem.254, 9943.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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