Saltar al contenido
Merck

Saltar a

345386

Glucose Oxidase, Aspergillus niger, Recombinant

Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid.

Sinónimos:

Glucose Oxidase, Aspergillus niger, Recombinant

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
A ustedes/SKUDisponibilidadPrecio

Acerca de este artículo

Número CAS:
Número CE:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Specific activity:
≥250 units/mg solid
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle


Quality Segment

description

Merck USA index - 14, 4460

form

lyophilized solid

specific activity

≥250 units/mg solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

yellow-brown

solubility

0.1 M phosphate buffer, pH 7.0: 5 mg/mL

foreign activity

Amylase ≤0.1%, catalase ≤20 U/mg (dry weight), sucrase ≤0.1%

shipped in

ambient

storage temp.

2-8°C

SMILES string

O1[C@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O)O)O)O

InChI

1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1

InChI key

WQZGKKKJIJFFOK-VFUOTHLCSA-N

General description

Note: 1 KU = 1000 units.
Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Each molecule of enzyme contains 2 molecules of FAD, which are essential for enzyme activity. Optimal pH = 6.5 with >95% maximal activity at pH 6.0-7.0. Useful for enzymatic assay of glucose. May also be conjugated to antibodies or antigens for use in ELISA procedures.

Preparation Note

Following reconstitution, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Heinz, F. and Beushausen, T.W. 1981. J. Clin. Chem. Clin. Biochem. 19, 977.
One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
G2133G7141172426
specific activity

≥250 units/mg solid

specific activity

≥100,000 units/g solid (without added oxygen)

specific activity

100,000-250,000 units/g solid (without added oxygen)

specific activity

≥10 unit/mg solid, ≥20 units/mg protein

form

lyophilized solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

ambient

shipped in

wet ice

shipped in

-

shipped in

wet ice

solubility

0.1 M phosphate buffer, pH 7.0: 5 mg/mL

solubility

-

solubility

-

solubility

aqueous buffer: 1 mg/mL, water: 1 mg/mL

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

OK to freeze


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos



Questions

1–2 of 2 Questions  
  1. Hello! We are looking at recombinant glucose oxidase from Aspergillus Niger, 345386. Does this contain a His-tag for purification? Also could you please provide an estimate for the mass, based on a given activity. What is the approximate mass of 10 KU? 

    1 answer
    1. The approximate mass of this product will depend on the specific activity. According to the product specifications, the activity is ≥250 units/mg solid, and the product is supplied in a 10 KU pack size. For a lot with 250 units/mg, the approximate mass will be 40 mg. Please check the certificate of analysis for the actual activity of each lot. This recombinant protein does not contain any epitope tag for purification purposes.

      Helpful?

  2. what is the application of Recombinant Glucose Oxidase sigma? can use it in medical?

    1 answer
    1. This product is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors. It is useful for enzymatic determination of glucose concentrations in various samples. Unless otherwise stated this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.

      It may be helpful to review the following publications below:
      https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.2165
      https://academic.oup.com/lambio/article-abstract/58/4/393/6699633?redirectedFrom=fulltext

      Helpful?

Reviews

No rating value

Active Filters