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05-1466

Sigma-Aldrich

Anti-FOXA1 Antibody, clone 2F83

clone 2F83, from mouse

Sinónimos:

Forkhead box protein A1, forkhead box A1, hepatocyte nuclear factor 3, alpha

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

clone

2F83, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

General description

FOXA1 (HNF3A, Hepatocyte nuclear factor 3-alpha) is member of the Forkhead family of winged-helix transcription factors. It is known to be involved in the development and differentiation of several organs including liver, kidney, pancreas, lung, prostate and mammary gland (Lupien, 2008). High expression of FOXA1 is often seen in tumors arising from both the prostate and breast where it is believed to interact with either the androgen receptor or estrogen receptor α, in each of the tumor type respectively (Lupien, 2008). In the case of ER, FOXA1 has gained notice because of its interactions with the cis-regulatory regions in heterochromatin and because it enhances the interaction of ER with chromatin (Badve, 2007).

Specificity

Other species have not been tested.
This antibody detects human FOXA1 (HNF3α).

Immunogen

Epitope: N-terminus
Recombinant human GST-FOXA1 protein encompassing amino acids 7-86.

Application

Immunohistochemisty:
Independent labs have verified this clone to work in paraffin-embedded IHC (IH(P)) of various mouse tissues including both embryonic and adult brain, adult digestive tract, adult respiratory tract, and adult urinary tract. Staining was performed using the antibody at a 1:2,000 dilution, but dilutions and conditions must be user optimized.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-FOXA1 Antibody, clone 2F83 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXA1 also known as Forkhead box protein A1, hepatocyte nuclear factor 3 alpha.

Quality

Evaluated by western blot on HeLa nuclear extracts.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect FOXA1 in HeLa nuclear extract.

Target description

49 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Heba Sheta et al.
Pathologica, 113(2), 102-114 (2021-05-28)
Forkhead box (FOX) A1 is a potential therapeutic biomarker that has been investigated in various human cancers. Limited data exist about FOXA1 biologic role in epithelial ovarian cancer (EOC). This study assessed FOXA1 immunohistochemical (IHC) expression and evaluated its association
Yawei Liu et al.
Nature communications, 8, 14709-14709 (2017-04-25)
Neurons reprogramme encephalitogenic T cells (Tenc) to regulatory T cells (Tregs), either FoxP3+Tregs or FoxA1+Tregs. We reported previously that neuronal ability to generate FoxA1+Tregs was central to preventing neuroinflammation in experimental autoimmune encephalomyelitis (EAE). Mice lacking interferon (IFN)-β were defective

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