M7023

Anti-Mouse IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162638
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i), QPA
• Citations: 49

Properties

• biological source: rabbit
• Quality Segment: 300
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): indirect ELISA: 1:80,000, quantitative precipitin assay: 2.0 mg/mL
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) is a member of the immunoglobulin family and contains gamma (γ) heavy chain in the constant (C) region. Monomeric structure of IgG contains two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds implicated in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3 and IgG4 with different heavy chains, named γ1, γ2, γ3 and γ4, respectively.

Immunogen

Anti-Mouse IgG is produced in rabbit using purified mouse IgG as the immunogen. Whole antiserum is purified to provide an IgG fraction of antiserum.

Application

Anti-Mouse IgG (whole molecule) antibody produced in rabbit was used as secondary antibody in immunoblotting, immunoprecipitation and immunohistochemistry.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

The antiserum is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP) versus normal mouse serum and mouse IgG. By Ouchterlony Double Diffusion (ODD) the product reacts with mouse IgG, mouse IgA and IgM.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)