M5899

Anti-Mouse IgG (whole molecule) antibody produced in goat

whole antiserum

• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162638
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i), QPA
• Citations: 12

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• technique(s): indirect ELISA: 1:70,000, quantitative precipitin assay: 3.2-4.8 mg/mL
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Application

Anti-Mouse IgG (whole molecule) antibody produced in goat has been used as secondary antibody in:

• immunoblotting
• immunohistochemistry
• proteomic microarrays

Anti-Mouse IgG (whole molecule) antibody produced in goat has been used in:

• the preparation of immunochromatography strips for magneto-enzyme lateral flow immunoassay[1]
• immunochromatography[1]
• MYCN DNA-binding analysis (ChIP-seq)[2]

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Biochem/physiol Actions

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Immunoglobulin G (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

The antiserum is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP) against normal mouse serum and purified mouse IgG.

Physical form

Supplied as a liquid containing 15 mM sodium azide as preservative.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Analysis Note

The antiserum has been treated to remove lipoproteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable