GC Analysis of Tricyclic Antidepressants in Urine on a 100% Methyl Column after SPME using 100 μm PDMS Fiber
Materiali
Colonna analitica
N° Catalogo
Descrizione
Determinazione del prezzo
Fibra per SPME
N° Catalogo
Descrizione
Determinazione del prezzo
Unità fibra SPME polidimetilsilossano (PDMS)
100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, plain red hubCONDITIONS
sample/matrix
1 mL urine (1 μg each analyte + 50 μL 5 M NaOH) in 7.5 mL vial
SPME fiber
polydimethylsiloxane, 100 μm (57300-U)
extraction
headspace, 15 min, 100 °C (sample incubated 30 min)
desorption process
3 min, 280 °C
column
100% methyl polysiloxane, 30 m × 0.32 mm I.D., 0.25 μm (Supelco equivalent, Equity-1, 28055-U)
oven
100 °C to 300 °C at 20 °C/min
inj. temp.
splitless (splitter opened after 1 min), 280 °C
detector
FID, 280 °C
carrier gas
helium, 30 cm/sec