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V900483

Sigma-Aldrich

Trizma® base

≥99%, crystalline powder, reagent grade, Vetec™

Sinonimo/i:

2-ammino-2-(idrossimetil)-1,3-propandiolo, THAM, Tris base, Tris(idrossimetil)amminometano, Trometamolo

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About This Item

Formula condensata:
NH2C(CH2OH)3
Numero CAS:
77-86-1
Peso molecolare:
121.14
Beilstein:
741883
Numero CE:
201-064-4
Numero MDL:
MFCD00004679
Codice UNSPSC:
12161700
ID PubChem:
329829884
Prezzi e disponibilità al momento non sono disponibili

Nome del prodotto

Trizma® base, Vetec™, reagent grade, ≥99%

Grado

reagent grade

Descrizione

aminopeptidase substrate

Nome Commerciale

Vetec™

Saggio

≥99%

Stato

crystalline powder

pH

10.5-12

Intervallo di pH utile

7-9

pKa (25 °C)

8.1

P. ebollizione

219-220 °C/10 mmHg (lit.)

Punto di fusione

167-172 °C (lit.)

Solubilità

water: 0.667 g/mL, clear, colorless to faintly yellow

Stringa SMILE

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
LENZDBCJOHFCAS-UHFFFAOYSA-N

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Descrizione generale

Trizma® base is a basimetric standard used in biochemistry and molecular biology. It may be used as a buffer or as a component of mixed buffer formulations. It is essentially stable, relatively non-hygroscopic and has a high equivalent weight.[1][2]

Applicazioni

Trizma® base can be used as a buffer:
  • In the evanescent absorption analysis of NADPH.[3]
  • In the autoxidation studies of oxymyoglobin.[4]

Altre note

I valori di pH di tutti i tamponi dipendono dalla temperatura e dalla concentrazione. Per i tamponi Tris, ad ogni °C in meno della temperatura corrisponde un aumento di circa 0,03 unità di pH, mentre ad ogni diluizione di un fattore 10 corrisponde una diminuzione di pH di 0,03-0,05 unità.
Per applicazioni che necessitano estrema precisione, utilizzare un pHmetro accuratamente calibrato con un elettrodo combinato vetro/calomelano.

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
WXBF5258V
WXBF2513V
WXBF4131V
WXBF2803V
WXBF2802V

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Tapered fibers for cell studies
Reviews in Fluorescence (2005)
Preparation of buffers for use in enzyme studies
Handbook of Biochemistry and Molecular Biology (2010)
Autoxidation of native oxymyoglobin. Kinetic analysis of the pH profile
Shikama K and Sugawara Y
European Journal of Biochemistry, 91(2), 407-413 (1978)
Tri (hydroxymethyl) aminomethane as Acidimetric Standard.
Fossum J, et al.
Analytical Chemistry, 23(3), 491-493 (1951)
Sylvain Clède et al.
Chemical communications (Cambridge, England), 48(62), 7729-7731 (2012-06-29)
A rhenium tris-carbonyl derivative has been designed to couple infrared and luminescent detection in cells. Both spectroscopies are consistent with one another; they point out the reliability of the present SCoMPI (for Single Core Multimodal Probe for Imaging) for bimodal
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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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