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66905-U

Supelco

BIOshell A160 Peptide C18 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

Sinonimo/i:

Superficially Porous Wide Pore C18 UHPLC Column

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

Nome del prodotto

BIOshell A160 Peptide C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

Materiali

stainless steel hardware

Livello qualitativo

agenzia

suitable for USP L1

Descrizione

Shell thickness (0.5 μm)
Solid Core (1.7 μm)

Nome Commerciale

BIOshell
BIOshell

Caratteristiche

endcapped: no

Classi chimiche degli analiti

peptides
peptides

Produttore/marchio commerciale

BIOshell
BIOshell

Confezionamento

1 ea of

Grado di funzionalizzazione

4.6% carbon loading

Parametri

600 bar max. pressure
90 °C max. temp.

tecniche

HPLC: suitable
HPLC: suitable
LC/MS: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
UHPLC: suitable

Lungh. × D.I.

15 cm × 2.1 mm

Area superficiale

90 m2/g

Copertura della superficie

2.2 μmol/m2

Matrice

spherical silica particle platform
superficially porous particle
superficially porous particle

Gruppo funzionale matrice

C18 (octadecyl) bonding phase, diisobutyloctadecyl

Dimensione particelle

2.7 μm
2.7 μm

Dimensione pori

160 Å

Intervallo di pH d’impiego

1-8

Tecnica di separazione

reversed phase
reversed phase

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Descrizione generale

BIOshell 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected C18 ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C). This greatly expands the application range for the separation of biomolecules.

Applicazioni

BIOshell A160 Peptide C18, 2.7 μm HPLC Column, a non-end capped column that has superficially porous particle with C18 alkyl ligand as the stationary phase, is used mainly in the separation of peptides at a good flow rate.

Caratteristiche e vantaggi

BIOshell A160 Peptide C18, 2.7 μm HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than columns with fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

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Note legali

Precolonna

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Journal of functional foods, 46, 456-462 (2018)
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The Peptide RPC Column Characterisation Protocol was applied to 38 stationary phases, varying in ligand chemistry, base silica, end capping and pore size, which are suitable for the analysis of peptides. The protocol at low and intermediate pH is based

Articoli

Learn about UHPLC-MS analysis of trastuzumab on a BIOshell™ 160A peptide C18 column and discover reduced solvent consumption in peptide mapping using 1.5 mm I.D. columns.

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

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