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53804-U

Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 7.5 cm × 2.1 mm, HPLC Column

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Prezzi e disponibilità al momento non sono disponibili

Nome del prodotto

Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 7.5 cm × 2.1 mm

Materiali

stainless steel column

Livello qualitativo

100

agenzia

suitable for USP L1

Nome Commerciale

Ascentis®

Caratteristiche

endcapped

Produttore/marchio commerciale

Ascentis®

Confezionamento

1 ea of

Parametri

60 °C temp. range
600 bar max. pressure (9000 psi)

tecniche

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

Lungh. × D.I.

7.5 cm × 2.1 mm

Area superficiale

135 m2/g

Impurezze

<5 ppm metals

Matrice

Fused-Core particle platform
superficially porous particle

Gruppo funzionale matrice

C18 (octadecyl) phase

Dimensione particelle

2.7 μm

Dimensione pori

90 Å

pH di lavoro

2-9

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Categorie correlate

Descrizione generale

Le colonne per HPLC Ascentis Express, grazie alla tecnologia delle particelle Fused-Core®, offrono l′alta velocità e l′elevata efficienza delle particelle di dimensioni inferiori a 2 μm, mantenendo ridotte nel contempo le contropressioni. La combinazione di alta efficienza e bassa contropressione è un grande vantaggio per tutti coloro che utilizzano la UPLC® (o altri sistemi a pressione ultra-alta) e l′HPLC convenzionale.
Per ulteriori informazioni su questa nuova tecnologia, si rimanda alla pagina Ascentis Express

Applicazioni

Analisi HPLC delle capsaicine contenute nel peperoncino con la colonna Ascentis® Express C18, 2,7 μm
Analisi UHPLC/MS dell′olio di mais con la colonna Ascentis® Express C30 confrontata con Ascentis® Express C18
Analisi UHPLC del paracetamolo e delle sue impurezze con la colonna Ascentis® Express C18, 2,7 μm
Analisi HPLC dei parabeni con la colonna Ascentis®Express C18, 2,7 μm

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

Precolonna

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Prodotti correlati

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Descrizione
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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
USMA002263
USMA002262
USMA002261
USMA002260
USMA002259

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Silvia Jakabová et al.
Journal of chromatography. A, 1232, 295-301 (2012-03-07)
Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a
Petra Šilarová et al.
Food chemistry, 237, 471-480 (2017-08-03)
The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Judy Stone
Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)
Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
Visualizza tutti i documenti correlati

Articoli

Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

Protocolli

Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3

We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.

Questions

1–9 of 9 Questions  

  1. Can I use Ascentis Express on a UHPLC system?

    1 answer

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  2. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 answer

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Helpful?

  3. Can I use Ascentis Express on any type of HPLC system?

    1 answer

    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 answer

    1. Nothing special is required to use Ascentis Express HPLC columns.  To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.  For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).

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  6. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 answer

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

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  7. What is the carbon load for Ascentis® Express C18 HPLC Columns?

    1 answer

    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared

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  8. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 answer

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      Helpful?

  9. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 answer

    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

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