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Y1771

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

Sinonimo/i:

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements

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20 G
CHF 166.00

CHF 166.00

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20 G
CHF 166.00

About This Item

Numero MDL:
MFCD01095679
Codice UNSPSC:
41106212
NACRES:
NA.85

CHF 166.00

Check Cart for Availability

Livello qualitativo

200

Stato

powder

tecniche

transformation: suitable

applicazioni

microbiology

Temperatura di conservazione

room temp

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Categorie correlate

Applicazioni

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

  • the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids[1]
  • Yarrowia lipolytica PO1f strain positive transformants[2]
  • CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants[3]

Altre note

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Nota sulla preparazione

Dissolve 1.46 g of supplement per liter of medium.

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Y2146

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, and tryptophan

Pittogrammi

Corrosion
GHS05

Avvertenze

Danger

Indicazioni di pericolo

H318

Consigli di prudenza

P280 - P305 + P351 + P338

Classi di pericolo

Eye Dam. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000378624
0000350645
0000350645
0000378624
0000300684

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Emil D Jensen et al.
Bio-protocol, 12(5), e4346-e4346 (2022-05-21)
Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants with improved traits from vast mutated sequence spaces.
Paul Swietalski et al.
Yeast (Chichester, England), 39(3), 230-240 (2021-10-15)
Orotic acid (OA) is an intermediate of the pyrimidine biosynthesis with high industrial relevance due to its use as precursor for production of biochemical pyrimidines or its use as carrier molecule in drug formulations. It can be produced by fermentation
Paul Swietalski et al.
Microbial cell factories, 19(1), 100-100 (2020-05-13)
The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of the secretory pathway. Therefore, correct folding, maturation and intracellular transport of the recombinant proteins are important processing steps

Protocolli

Yeast Drop Out

Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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