WGA1
GenomePlex® Whole Genome Amplification (WGA) Kit
Kit for whole genome amplification from a variety of DNA sources including FFPE tissue
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
41121800
NACRES:
NA.55
Prodotti consigliati
tecniche
whole genome amplification: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
GenomePlex® Whole Genome Amplification WGA kit is suitable for use with genomic DNA, whole blood, buffy coats, buccal swabs, and cultured cells. Amplification requires only a small amount of starting material (1 μL blood, single cheek swab, or 10 ng of gDNA), which after PCR produces a yield of 5 - 10 μg per reaction. The amplified DNA can be purified and stored or used for downstream analyses including ABI′s TaqMan® assays, microsatellite analysis, or sequencing. GenomePlex gives a complete representation of the entire genome with no detectable allele bias.
WGA Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.
GenomePlex method allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The amplification yield is dependent on the purity and amount of starting material. This kit contains all the required reagents to perform library preparation and fragmentation.
WGA Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.
GenomePlex method allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The amplification yield is dependent on the purity and amount of starting material. This kit contains all the required reagents to perform library preparation and fragmentation.
Applicazioni
GenomePlex® Whole Genome Amplification (WGA) Kit has been used to amplify input and chromatin immunoprecipitation (ChIP) DNA. It has also been used in the fragmentation of bacterial artificial chromosome (BAC) clones from bacterial cultures for the preparation of repeat free (RF) probes.
Caratteristiche e vantaggi
- Higher yield from minimal template: Amplification of nanogram amounts (10ng) of genomic DNA to microgram yields (5-10 μg) in less than about three hours
- Nanograms of samples can be preserved at –20 °C for future use
- Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, and formalin-fixed paraffin-embedded tissue (FFPE)
- Compatible with a wide variety of downstream applications such as TaqMan® and BeadArray assays
- Universal primers
- Whole-genome representation with no detectable bias
- Increased accuracy in amplification produces no amplicon in the negative control reactions
Altre note
The sequences of the universal primers provided in this kit are considered proprietary.
Note legali
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- W4502Water, Nuclease-Free Water, for Molecular BiologySDS
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Binding patterns of BCL11A in the globin and GATA1 loci and characterization of the BCL11A fetal hemoglobin locus
Jawaid K, et al.
Blood Cells, Molecules and Diseases, 45(2), 140-146 (2010)
Jessica Ray et al.
PloS one, 7(4), e34238-e34238 (2012-04-18)
Viruses are ubiquitous in the oceans and critical components of marine microbial communities, regulating nutrient transfer to higher trophic levels or to the dissolved organic pool through lysis of host cells. Hydrothermal vent systems are oases of biological activity in
Ravinder Dhallan et al.
Lancet (London, England), 369(9560), 474-481 (2007-02-13)
Use of free fetal DNA to diagnose fetal chromosomal abnormalities has been hindered by the inability to distinguish fetal DNA from maternal DNA. Our aim was to establish whether single nucleotide polymorphisms (SNPs) can be used to distinguish fetal DNA
Agnieszka Maciejewska et al.
International journal of legal medicine, 127(2), 309-319 (2012-09-04)
Degraded DNA is often analyzed in forensic genetics laboratories. Reliable analysis of degraded DNA is of great importance, since its results impact the quality and reliability of expert testimonies. Recently, a number of whole genome amplification (WGA) methods have been
Whole genome amplification and genetic analysis after extraction of proteins from dried blood spots.
Mads V Hollegaard et al.
Clinical chemistry, 53(6), 1161-1162 (2007-05-23)
Articoli
Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.
Protocolli
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
Contenuto correlato
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.