The optimal pH for this enzyme ranges from 3.3 to 5.5, and the optimal temperature is between 40 to 50 °C. For stock solution preparation, 30 mM PBS pH 5.0 may be used for reconstitution.
V2010
Viscozyme® L
cellulolytic enzyme mixture
Sinonimo/i:
Cell Wall Degrading Enzyme Complex from Aspergillus sp., Lysing Enzyme from Aspergillus sp.
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
50 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 116.00 |
250 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 320.00 |
Informazioni su questo articolo
Biological source:
fermentation/recombinant
Servizio Tecnico
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Permettici di aiutartibiological source
fermentation/recombinant
Quality Segment
form
liquid
enzyme activity
≥100 FBGU/g
technique(s)
protein extraction: suitable
density
~1.2 g/mL at 25 °C (lit.)
suitability
suitable for cell lysis
application(s)
cell analysis
storage temp.
2-8°C
General description
Viscozyme® L is a lignocellulose-specialized multi-enzymatic complex derived from Aspergillus aculeatus. It contains a wide range of carbohydrases, including arabanase, cellulase, β-glucanase, hemicellulase, and xylanase.
Application
Viscozyme® L has been used:
- in the extraction of polyphenol from grapefruit and unripe apples.
- to study the effect of its supplementation on nutritive value and silage characteristics of sunflower silage.
- for simulation of the fermentation process during in vitro digestibility of former food products (FFPs).
- for enzymatic treatment, to study its effects on the antioxidant properties of wheat bran.
Biochem/physiol Actions
Viscozyme L aids in the release of oil bodies and proteins from cells by degrading the hemicellulose, cellulose, and pectin molecules, and by breaking the cell wall components.
Features and Benefits
- Not a GMO and doesn′t contain GMOs.
- Made by fermentation of a microorganism.
- Improves process through faster, more efficient oil/water separation.
Legal Information
A product of Novozyme Corp.
Viscozyme is a registered trademark of Novozymes Corp.
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Questo articolo | |||
|---|---|---|---|
| description cellulolytic enzyme mixture | description - | description Type XXIII, ≥3 units/mg solid | description ≥8.0 units/mg solid |
| biological source fermentation/recombinant | biological source - | biological source Aspergillus sp. (A. melleus) | biological source - |
| technique(s) protein extraction: suitable | technique(s) - | technique(s) - | technique(s) - |
| suitability suitable for cell lysis | suitability - | suitability - | suitability - |
| application(s) cell analysis | application(s) - | application(s) - | application(s) - |
| form liquid | form - | form solid | form solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
flash_point_f
Not applicable
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 1
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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What is the optimum pH and Temperature of this enzyme?
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What is the concentration I should use?
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For the reduction of beta-glucans and viscosity, a dosage of 0.02-0.1% of grist weight is recommended. For other applications, a general recommendation is 0.05-0.1%.
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What does the hemicellulases of this product contain? Can it degrade mannan or galactan?
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The key enzyme activity is endo-beta-glucanase, which hydrolyzes (1,3)- or (1,4)-linkages in beta-D-glucans. This product contains side activities of xylanase, cellulase, and hemicellulose. Mannan or galactan degradation is not expected.
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What's the cas number of Viscozyme L?
1 answer-
A CAS Registry Number is assigned to specific compounds. This product is a blend of beta-glucanases, pectinases, hemicellulases and xylanases and is not assigned a CAS number.
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What enzymes does this product contain exactly? And does the ability to degrade pectin mean this product contains pectinase? And what type of hemicellulase does this product contain?
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This product is a blend of beta-glucanases, pectinases, hemicellulases, and xylanases.
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How many units of enzyme in this 50ml?
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The unit value of this product is lot specific and reported on the Certificate of Analysis. A survey of the most recent lots shows and average activity of 108 units per gram and a density of 1.21 g/ml. Please see the link below to access the lot specific Certificate:
https://www.sigmaaldrich.com/searchHelpful?
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