Unfortunately, there is no protocol available for this product. However, see the link below for a publication that may be helpful
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760808/
SRP6371
Aldose Reductase human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Sinonimo/i:
ADR, AKR1B1, ALDR1, ALR2, AR, Aldo-keto reductase family1, member B1
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About This Item
Codice UNSPSC:
12352204
NACRES:
NA.32
Prodotti consigliati
Origine biologica
human
Ricombinante
expressed in E. coli
Saggio
≥95% (SDS-PAGE)
Stato
liquid
PM
35.8 kDa (316 aa, 1-316 aa)
Confezionamento
pkg of 100 μg
Concentrazione
1 mg/mL
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Informazioni sul gene
human ... AKR1B1(231)
Descrizione generale
Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily. Recombinant aldose reductase (AKR1B) protein was expressed in Escherichia coli and purified by using conventional chromatography techniques. It is expressed in eye and kidney, but predominant in placenta.[1] The AKR1B1 gene is mapped to human chromosome 7q33.[2]
Azioni biochim/fisiol
Aldose reductase (AKR1B1) catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.[3][4] AKR1B1 is also involved in cytokine-mediated generation of prostaglandin F2α (PGF2α) in preadipocytes.[5] It participates in diabetes-associated mitochondrial disorder and damage via the activation of p53.[6] AKR1B1 is associated with drug resistance mechanism in cancer-derived cell lines. It is also known to mediate cellular processes linked to carcinogenesis such as EMT (epithelial–mesenchymal transition), inflammation and angiogenesis.[7] Aldose reductase mediates glucose metabolism in placenta and osmotic control in eye and kidney.[1]
Stato fisico
1 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Expression of the Aldo-Ketoreductases AKR1B1 and AKR1B10 in Human Cancers.
Laffin B and Petrash JM
Frontiers in Pharmacology, 3, 104-104 (2012)
The crystal structure of the aldose reductase.NADPH binary complex.
Borhani DW, et al.
The Journal of Biological Chemistry, 267, 24841-24847 (1992)
Is rs759853 polymorphism in promoter of aldose reductase gene a risk factor for diabetic nephropathy? A meta-analysis.
Cui W, et al.
European Journal of Medical Research, 20, 14-14 (2015)
The human aldose reductase AKR1B1 qualifies as the primary prostaglandin F synthase in the endometrium.
Bresson E, et al.
The Journal of Clinical Endocrinology and Metabolism, 96, 210-219 (2011)
Prostaglandin (PG) F2 alpha synthesis in human subcutaneous and omental adipose tissue: modulation by inflammatory cytokines and role of the human aldose reductase AKR1B1.
Michaud A, et al.
PLoS ONE, 9, e90861-e90861 (2014)
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