SRP3252

SDF-1 alpha from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Sinonimo/i: CXCL12, PBSF, Stromal-Cell Derived Factor-1
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: rat
• Ricombinante: expressed in E. coli
• Saggio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Stato: lyophilized
• Potenza: 50.0-100.0 ng/mL
• PM: 7.9 kDa
• Confezionamento: pkg of 10 μg
• tecniche: cell culture | mammalian: suitable
• Impurezze: <0.1 EU/μg endotoxin, tested
• Colore: white
• N° accesso UniProt: Q9QZD1
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: rat ... CXCL12(24772)

Descrizione

Descrizione generale

Stromal cell-derived factor 1 α and β (SDF-1α and β) are stromal derived CXC chemokines, and signal through the C-X-C motif chemokine receptor-4 (CXCR4).[1] SDF-1α is also known as C-X-C motif chemokine ligand 12 (CXCL12). Recombinant rat SDF-1α is a 7.9kDa protein containing 68 amino acid residues.

Applicazioni

Stromal cell-derived factor 1 α (SDF-1α ) has been used in cell migration assay and fibrin gel bead assay.[2]

Azioni biochim/fisiol

Stromal cell-derived factor 1 α and β (SDF-1α and β) chemoattract B and T cells, and have been shown to induce migration of CD34⁺ stem cells.[3][4] Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor. SDF-1 α modulates leukocyte and hematopoietic precursor migration and pre-B cell proliferation.[5]

Sequenza

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

Stato fisico

Lyophilized with no additives.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable