SAE0091
Lisostafina
free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research
Sinonimo/i:
Glicilglicina endopeptidasi
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Cambia visualizzazione
2 MG
CHF 228.00
About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.54
Prodotti consigliati
Nome del prodotto
Lisostafina, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein
Origine biologica
microbial (Staphylococcus staphylotyticus)
Stato
lyophilized powder
Attività specifica
≥500 units/mg protein
PM
27 kDa
Caratteristiche
DNA free
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
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Applicazioni
Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)
Azioni biochim/fisiol
La lisostafina è una endopeptidasi zinco dipendente con un peso molecolare di circa 25 kDa. La lisostafina scinde i legami crociati della poliglicina nello strato di peptoglicano della parete cellulare di specie di Staphylococcus ; è risultata quindi utile per la lisi cellulare oltre che come potenziale agente terapeutico antimicrobico.
pH ottimale per l′attività: circa 7,5
pH ottimale per l′attività: circa 7,5
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.
Definizione di unità
One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.
Stato fisico
Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
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