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S5134

Sigma-Aldrich

Scott′s Tap Water Substitute Concentrate

10 ×

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About This Item

Codice UNSPSC:
41116124
NACRES:
NA.47
Prezzi e disponibilità al momento non sono disponibili

Stato

powder

Livello qualitativo

IVD

for in vitro diagnostic use

Concentrazione

10 ×

tecniche

titration: suitable

pH

7.0-7.8

applicazioni

hematology
histology

Temperatura di conservazione

room temp

Applicazioni

Used as a "blueing reagent" in hematoxylin and eosin staining procedures.[1] Scott′s Tap Water Substitute Concentrate has been used in transferase−mediated dUTP nick end labeling (TUNEL) assay.[2] It has been used in immunohistochemical analysis.[3]

Altre note

MgSO4 • 7H2O, 200 g/L + NaHCO3, 20 g/L

Nota sulla preparazione

Scott′s Tap Water Substitute is prepared by mixing 1 part of Scott′s Tap Water Substitute Concentrate with 9 parts deionized water.

Pittogrammi

Exclamation markEnvironment

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves


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Questions

1–3 of 3 Questions  
  1. Product 65354-85 has been deleted. What is a suitable replacement product?

    1 answer
    1. Suitable replacements for the deleted Blueing Reagent (65354-85) are S5134 and 106329. Scott's Tap Water Concentrate is diluted 1 to 10 before use. 106329 is typically dissolved in water at a concentration of 15 grams per liter. It is also possible to use a 0.2% solution of ammonium hydroxide or a saturated solution of lithium carbonate.

      Helpful?

  2. Must Scott's Tap Water Substitute be diluted with deionized water, and must the solution be prepared fresh for each day?

    1 answer
    1. The Scott's Tap Water Substitute Concentrate should be diluted with deionized water. When using distilled water, it is advisable to check the pH after preparing the 1X solution. Alternatively, the solution prepared with distilled water can be used to blue tissue sections after staining with hematoxylin. If the nuclei do not turn blue, it may indicate that the pH of the solution is not alkaline enough to convert the nuclei color to blue.

      Helpful?

  3. Is there a direct replacement for S5134 Scott's Tap Water Substitute?

    1 answer
    1. There is no direct replacement for the product in question. However, a solution with 15 grams of sodium bicarbonate per liter of water serves exactly the

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