P7255

Puromicina

≥98% (HPLC), powder

• Sinonimo/i: 3′-[α-ammino-p-metossiidrocinnamamido]-3′-desossi-N,N-dimetiladenosina
• Formula empirica (notazione di Hill): C₂₂H₂₉N₇O₅ · 2HCl
• Numero CAS: 58-58-2
• Peso molecolare: 544.43
• UNSPSC Code: 51281912
• NACRES: NA.77
• PubChem Substance ID: 57654556
• EC Number: 200-387-8
• Beilstein/REAXYS Number: 3853613
• MDL number: MFCD00012691
• Assay: ≥98% (HPLC)
• Form: powder

Proprietà

• Quality Segment: 300
• assay: ≥98% (HPLC)
• form: powder
• greener alternative product score: old score: 88; new score: 79; Find out more about DOZN™ Scoring
• greener alternative product characteristics: Atom Economy; Design for Energy Efficiency; Use of Renewable Feedstocks; Design for Degradation; Learn more about the Principles of Green Chemistry.
• sustainability: Sustainability features
• solubility: H₂O: 50 mg/mL
• antibiotic activity spectrum: Gram-positive bacteria, neoplastics, parasites
• mode of action: protein synthesis | interferes
• storage temp.: −20°C
• SMILES string: Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1
• InChI: 1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
• InChI key: MKSVFGKWZLUTTO-FZFAUISWSA-N

Descrizione

General description

Chemical structure: peptidyl nucleoside

Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Atom Economy”, “Design for Energy Efficiency”, “Use of Renewable Feedstock” and “Design for Degradation”. Click here to view its DOZN scorecard.

Application

Recommended for use as a selection agent at a range of 1-10 μg/mL.

Biochem/physiol Actions

Modalità d′azione: La puromicina inibisce la sintesi proteica provocando l′arresto prematuro della catena con funzione di analogo dell′estremità 3′-terminale dell′aminoacil-tRNA.

Modalità di resistenza: La puromicina acetiltransferasi è un gene di resistenza efficace.

Spettro antimicrobico: Questo prodotto è attivo nei confronti dei microrganismi gram positivi, meno attivo nei confronti dei bacilli acido.rapidi e più debolmente attivo nei confronti dei microrganismi gram negativi. La puromicina può impedire la crescita di batteri, protozoi, alghe e cellule di mammifero e agisce rapidamente, uccidendo il 99% delle cellule entro 2 giorni.

Puromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger. It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL.

Features and Benefits

This compound is a featured product for Gene Regulation research. Click here to discover more featured Gene Regulation products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Preparation Note

This product is soluble in water at 50 mg/mL. A stock solution should be prepared by filtration using a 0.22 μm filter and then stored in aliquots at -20°C. This product is also soluble in methanol at 10 mg/mL.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.Keep in a dry place. Keep in a dry place

Disclaimer

As supplied, this product remains active for four years when stored at -20°C.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Parola di avvertenza: Warning
• Codici di pericolo: H302
• Avvertenze di sicurezza: P301 + P312 + P330
• Hazard Classifications: Acute Tox. 4 Oral
• Classe di stoccaggio: 11 - Combustible Solids
• Che succede?: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable
• DPI (dispositivi di protezione individuale): dust mask type N95 (US), Eyeshields, Gloves