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N7265

Sigma-Aldrich

Nitrate Reductase (NAD[P]H) from Aspergillus niger

lyophilized powder, ≥300 units/g solid

Sinonimo/i:

NAD(P)H:Nitrate oxidoreductase

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About This Item

Numero CAS:
9029-27-0
Classificazione EC (Enzyme Commission):
1.7.1.2 (BRENDA, IUBMB)
Numero MDL:
MFCD00131725
Codice UNSPSC:
12352204
NACRES:
NA.54

Forma fisica

lyophilized powder

Attività specifica

≥300 units/g solid

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

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Descrizione generale

Nitrate reductase (NAD[P]H) is expressed in variety of organisms including bacteria, fungi, algae, and higher plants which have capacity to use nitrate as their sole source of nitrogen.Experimental studies show that nitrate reductase (NAD[P]H) is 180,000Daltons.

Azioni biochim/fisiol

Nitrate Reductase (NAD[P]H) catalyzes the reduction of nitrate to nitrite. In addition, it also catalyzes the reduction of NADPH-cytochrome c, FADH2-nitrate. Nitrate reductase also has reduced methyl viologen-nitrate reductase activities.

Avvertenza

light and moisture sensitive

Definizione di unità

One unit will reduce 1.0 μmole of nitrate per min in the presence of β-NADPH at pH 7.5 at 25 °C.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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P C Allen et al.
Poultry science, 76(6), 822-827 (1997-06-01)
Two experiments were conducted to determine effects of diets containing n-3 fatty acids (n-3FA) from whole as well as ground flaxseed on the performance of broilers during coccidia infections. Diets were fed from 1 d of age through 3 wk
R L Warner et al.
Plant physiology, 91, 947-953 (1989-01-01)
Barley (Hordeum vulgare L.) has NADH-specific and NAD(P)H-bispecific nitrate reductase isozymes. Four isogenic lines with different nitrate reductase isozyme combinations were used to determine the role of NADH and NAD(P)H nitrate reductases on nitrate transport and assimilation in barley seedlings.
G Unden et al.
Molecular microbiology, 25(2), 205-210 (1997-07-01)
The FNR (fumarate and nitrate reductase regulation) protein of Escherichia coli is an oxygen-responsive transcriptional regulator required for the switch from aerobic to anaerobic metabolism. In the absence of oxygen, FNR changes from the inactive to the active state. The
W H Campbell
Cellular and molecular life sciences : CMLS, 58(2), 194-204 (2001-04-06)
Pyridine nucleotide-dependent nitrate reductases (NRs; EC 1.6.6.1-3) are molybdenum-containing enzymes found in eukaryotic organisms which assimilate nitrate. NR is a homodimer with an approximately 100 kDa polypeptide which folds into stable domains housing each of the enzyme's redox cofactors--FAD, heme-Fe
P Hilliquin et al.
Arthritis and rheumatism, 40(8), 1512-1517 (1997-08-01)
Nitric oxide (NO) is a free radical involved in inflammation and immune reactions. The presence of NO is usually assessed by assaying its degradation products, nitrite and nitrate. NO binds to thiol-containing proteins to form S-nitrosoproteins (S-NP). The aim of
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