M8320

Anti-MLH1 (N-terminal)

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-COCA2, Anti-FCC2, Anti-HNPCC, Anti-MGC5172
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• PM: antigen 80-85 kDa
• Reattività contro le specie: rat, human, mouse
• Confezionamento: antibody small pack of 25 μL
• Concentrazione: ~1 mg/mL
• tecniche: immunocytochemistry: 2.5-5 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton; immunoprecipitation (IP): 5-10 μg using Jurkat cell lysates; western blot: 0.5-1 μg/mL using Jurkat cell lysates
• N° accesso UniProt: P40692
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... MLH1(4292) ; mouse ... Mlh1(17350) ; rat ... Mlh1(81685)

Descrizione

Descrizione generale

MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.

MutL homolog 1 (MLH1) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors and signal transducers, named BRCA1-associated genome surveillance complex (BASC).

Immunogeno

synthetic peptide corresponding to amino acids 60-75 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse.

Applicazioni

• Anti-MLH1 (N-terminal) antibody produced in rabbit has been used in:
• immunoblotting
• immunoprecipitation
• immunocytochemistry

Azioni biochim/fisiol

MutL homolog 1 (MLH1) has been shown to be involved in stimulating carcinogenesis in the colon.

MutL protein (MLH), a homolog of the E. coli MutL gene, is involved in DNA mismatch repair. Nonpolyposis colorectal cancer-2 is caused by a hereditary mutation in the MLH1 gene. This cancer can also be a result of hypermethylation of one MLH1 allele in somatic cells (a germline epimutation).

Descrizione del bersaglio

MLH1 (N-terminal) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BASC (BRCA1-associated genome surveillance complex).

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)