04963
Lyticase
≥500 U/mL
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 g | Per conoscere la disponibilità, visualizza il carrello | CHF 40.10 |
5 g | Per conoscere la disponibilità, visualizza il carrello | CHF 162.00 |
Informazioni su questo articolo
Specific activity:
≥500 U/mL
CHF 40.10
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartiQuality Segment
form
liquid, viscous liquid
specific activity
≥500 U/mL
input
plant, fungi and insect sample(s)
solubility
H2O: 1:10, clear, colorless to very faintly green
suitability
corresponds for SDS-PAGE
storage temp.
−20°C
General description
Lyticase from Bacillus subtilis is a hydrolytic endoglycosidase that specifically cleaves β(1-3)-glucan linkages found in the cell walls and biofilm matrices of fungi and certain bacteria. Lyticase enzyme acts by degrading the structural glucan scaffold of microbial cell walls, enabling the generation of spheroplasts and facilitating nucleic acid extraction from yeast cells.
Application
Lyticase from Bacillus subtilis has been used for:
- digesting yeast cell walls to convert intact cells into spheroplasts enabling subsequent membrane isolation.
- enhancing antimicrobial treatment against interkingdom biofilms when combined with an enzymatic cocktail (cellulase, denarase, and dispersin B) prior to antimicrobial application.
Biochem/physiol Actions
Lyticase cleaves β(1-3) bonds within the glucose units of fungal cell walls and bacterial biofilm matrices, including the Psl polysaccharide of Pseudomonas aeruginosa breaking them down into smaller sugar fragments and dismantling the structural backbone of the cell. It dissolves the yeast (S. cerevisiae) cell wall, measurable as a decrease in optical turbidity at 800 nm (pH 7.5, 25°C). Lyticase requires two components to fully lyse cells: a glucanase that destroys the glucan scaffold, and an alkaline protease that removes the outer mannoprotein layer blocking glucanase access. By breaking down the C. albicans cell wall, lyticase increases permeability and enhances the killing effect of antifungal drugs such as caspofungin.
Other Notes
1U corresponds to the amount of enzyme which decreases the absorbance at 800 nm by 0.001 per minute at pH 7.5 and 25 C (Yeast, Cat. No. 51475, as substrate).
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Questo articolo | |||
|---|---|---|---|
| description ≥500 U/mL | description ≤1.00 EU/mg Endotoxin | description - | description ≥95% (HPLC) |
| specific activity ≥500 U/mL | specific activity - | specific activity - | specific activity - |
| suitability corresponds for SDS-PAGE | suitability - | suitability - | suitability - |
| form viscous liquid | form lyophilized powder | form solid | form powder |
| solubility H2O: 1:10, clear, colorless to very faintly green | solubility water: 9.80-10.20 mg/mL, hazy to turbid | solubility - | solubility ethanol: soluble 9.80-10.20 mg/mL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Parola di avvertenza
Danger
Codici di pericolo
Avvertenze di sicurezza
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
10 - Combustible liquids
Che succede?
WGK 1
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
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