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I3911

Sigma-Aldrich

Protease Inhibitor Cocktail

Animal-Free, lyophilized powder, for the inhibition of serine, cysteine, aspartic and metalloproteases, for general use, lyophilized powder

Sinonimo/i:

protease inhibitor

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1 PKG
CHF 94.60
5 X 1 PKG
CHF 351.00

CHF 94.60

Spedizione prevista il23 marzo 2025

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Cambia visualizzazione
1 PKG
CHF 94.60
5 X 1 PKG
CHF 351.00

About This Item

Codice UNSPSC:
12352200
NACRES:
NA.77
Stato:
lyophilized powder
Solubilità:
water: soluble
Temperatura di conservazione:
−20°C

CHF 94.60

Spedizione prevista il23 marzo 2025

Nome del prodotto

Protease Inhibitor Cocktail, Animal Component Free, for general use, lyophilized powder

Livello qualitativo

200

Stato

lyophilized powder

Solubilità

water: soluble

Temperatura di conservazione

−20°C

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Categorie correlate

Descrizione generale

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid-proteases, and aminopeptidases.

Specificità

Inhibits serine, cysteine, aspartic, and metalloproteases.

Applicazioni

Animal component free (ACF). Optimized and tested for general use. Protease Inhibitor Cocktail, Animal Component Free has been used to prevent protein lysis in saliva samples,[1] peritoneal residential macrophage cells[2] and in embryo culture medium.[3]

Componenti

AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin

Quantità

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Stato fisico

Lyophilized powder that is water soluble.
The components of this ACF cocktail do not originate from animal sources and can thus be used in the production of animal free proteins.

Pittogrammi

Corrosion
GHS05

Avvertenze

Danger

Indicazioni di pericolo

H314

Classi di pericolo

Eye Dam. 1 - Skin Corr. 1A

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
11190304
099M4056V
076M4124V
11160605
072M4051V

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Justin de Boer et al.
Journal of pediatric gastroenterology and nutrition, 71(4), e109-e112 (2020-09-23)
The gold standard diagnostic procedure for food protein-induced proctocolitis (FPIP) requires flexible sigmoidoscopy (FS). To date there is no validated, noninvasive test to confirm FPIP diagnosis. Eosinophil-derived neurotoxin (EDN), a product of eosinophil (EOS) degranulation, has been shown to correlate
Early protein profile of human embryonic secretome.
Foresta C, et al.
Frontiers in Bioscience, 21, 620-634 (2016)
Carlo Foresta et al.
Frontiers in bioscience (Landmark edition), 21, 620-634 (2015-12-29)
Embryos obtained by in vitro fertilization are currently assessed by morphology, but displays limitations with over 70% of embryos failing to implant. In this study, we performed HPLC-MS/MS analysis on the conditioned medium obtained from 50 human embryos at the
Marija Holcar et al.
Scientific reports, 10(1), 21346-21346 (2020-12-09)
Human plasma is a complex fluid, increasingly used for extracellular vesicle (EV) biomarker studies. Our aim was to find a simple EV-enrichment method for reliable quantification of EVs in plasma to be used as biomarker of disease. Plasma of ten
Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery
Lopucki M, et al.
Molecular Medicine Reports, 17(6), 8253-8259 (2018)
Visualizza tutti i documenti correlati

Contenuto correlato

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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